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Literature summary extracted from

  • Takahashi, S.; Yip, W.C.; Tamura, G.
    Purification and characterization of ferredoxin-sulfite reductase from turnip (Brassica rapa) leaves and comparison of properties with ferredoxin-sulfite reductase from turnip roots (1997), Biosci. Biotechnol. Biochem., 61, 1486-1490.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.7.1 cyanide 1 mM, added to the reduced enzyme Brassica rapa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.7.1 0.0028
-
 oxidized ferredoxin root enzyme Brassica rapa
1.8.7.1 0.013
-
 reduced ferredoxin leaf enzyme Brassica rapa
1.8.7.1 0.2
-
 sulfite root enzyme Brassica rapa
1.8.7.1 0.22
-
 sulfite leaf enzyme Brassica rapa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.7.1 64000
-
 1 * 64000, SDS-PAGE Brassica rapa
1.8.7.1 66000
-
 in presence of 200 mM NaCl, gel filtration Brassica rapa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.7.1 sulfite + reduced ferredoxin Brassica rapa
-
 hydrogen sulfide + oxidized ferredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.7.1 Brassica rapa
-
 cv. Hakutaka
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.7.1
-
 Brassica rapa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.7.1 leaf
-
 Brassica rapa
-
1.8.7.1 root
-
 Brassica rapa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.7.1 156
-
-
 Brassica rapa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.7.1 hydrogen sulfide + oxidized ferredoxin + H2O
-
 Brassica rapa sulfite + reduced ferredoxin + H+
-
 r
1.8.7.1 sulfite + reduced ferredoxin
-
 Brassica rapa hydrogen sulfide + oxidized ferredoxin + H2O
-
 ?
1.8.7.1 sulfite + reduced ferredoxin + H+
-
 Brassica rapa hydrogen sulfide + oxidized ferredoxin + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.7.1 monomer 1 * 64000, SDS-PAGE Brassica rapa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.7.1 60
-
 91% remaining activity of leaf enzyme after heating for 5 min, root enzyme lower heat stability Brassica rapa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.7.1 2.4
-
 sulfite root enzyme Brassica rapa
1.8.7.1 3.6
-
 reduced ferredoxin leaf enzyme Brassica rapa
1.8.7.1 6
-
 reduced ferredoxin root enzyme Brassica rapa
1.8.7.1 9.1
-
 sulfite leaf enzyme Brassica rapa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.7.1 7.8
-
 potassium phosphate buffer Brassica rapa

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.7.1 siroheme
-
 Brassica rapa