Literature summary extracted from
Reichenbecher, W.; Ruediger, A.; Kroneck, P.M.H.; Schink, B.
One molecule of molybdopterin guanine dinucleotide is associated with each subunit of the heterodimeric Mo-Fe-S protein transhydroxylase of Pelobacter acidigallici as determined by SDS/PAGE and mass spectrometry (1996), Eur. J. Biochem., 237, 406-413.
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.97.1.2 |
Fe |
iron-sulfur cluster |
Pelobacter acidigallici |
|
1.97.1.2 |
Mo |
enzyme contains Mo, 0.92 mol moybdenum per mol enzyme |
Pelobacter acidigallici |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.97.1.2 |
31300 |
- |
alpha,beta, 1 * 100400 + 1 * 31300, mass spectrometry |
Pelobacter acidigallici |
1.97.1.2 |
100400 |
- |
alpha,beta, 1 * 100400 + 1 * 31300, mass spectrometry |
Pelobacter acidigallici |
1.97.1.2 |
133000 |
- |
mass spectrometry |
Pelobacter acidigallici |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.97.1.2 |
Pelobacter acidigallici |
- |
large and small subunit |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.97.1.2 |
1,2,3,5-tetrahydroxybenzene + 1,2,3-trihydroxybenzene = 1,3,5-trihydroxybenzene + 1,2,3,5-tetrahydroxybenzene |
a mechanism is proposed which combines an oxidative hydroxylation with a reductive dehydroxylation via molybdenum cofactor |
Pelobacter acidigallici |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.97.1.2 |
1,2,3,5-tetrahydroxybenzene + pyrogallol |
pyrogallol i.e. 1,2,3-tri-hydroxybenzene |
Pelobacter acidigallici |
phloroglucinol + 1,2,3,5-tetrahydroxybenzene |
phloroglucinol i.e. 1,3,5-tri-hydroxybenzene |
? |
|
1.97.1.2 |
additional information |
water is not the source of the added hydroxyl groups |
Pelobacter acidigallici |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.97.1.2 |
heterodimer |
alpha,beta, 1 * 100400 + 1 * 31300, mass spectrometry |
Pelobacter acidigallici |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.97.1.2 |
molybdenum cofactor |
two molecules of molybdenum cofactor for each heterodimer, molybdenum is bound by a molybdopterin guanine dinucleotide |
Pelobacter acidigallici |
|