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Literature summary extracted from

  • Reichenbecher, W.; Ruediger, A.; Kroneck, P.M.H.; Schink, B.
    One molecule of molybdopterin guanine dinucleotide is associated with each subunit of the heterodimeric Mo-Fe-S protein transhydroxylase of Pelobacter acidigallici as determined by SDS/PAGE and mass spectrometry (1996), Eur. J. Biochem., 237, 406-413.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.97.1.2 Fe iron-sulfur cluster Pelobacter acidigallici
1.97.1.2 Mo enzyme contains Mo, 0.92 mol moybdenum per mol enzyme Pelobacter acidigallici

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.97.1.2 31300
-
alpha,beta, 1 * 100400 + 1 * 31300, mass spectrometry Pelobacter acidigallici
1.97.1.2 100400
-
alpha,beta, 1 * 100400 + 1 * 31300, mass spectrometry Pelobacter acidigallici
1.97.1.2 133000
-
mass spectrometry Pelobacter acidigallici

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.2 Pelobacter acidigallici
-
large and small subunit
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.97.1.2 1,2,3,5-tetrahydroxybenzene + 1,2,3-trihydroxybenzene = 1,3,5-trihydroxybenzene + 1,2,3,5-tetrahydroxybenzene a mechanism is proposed which combines an oxidative hydroxylation with a reductive dehydroxylation via molybdenum cofactor Pelobacter acidigallici

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.2 1,2,3,5-tetrahydroxybenzene + pyrogallol pyrogallol i.e. 1,2,3-tri-hydroxybenzene Pelobacter acidigallici phloroglucinol + 1,2,3,5-tetrahydroxybenzene phloroglucinol i.e. 1,3,5-tri-hydroxybenzene ?
1.97.1.2 additional information water is not the source of the added hydroxyl groups Pelobacter acidigallici ?
-
?

Subunits

EC Number Subunits Comment Organism
1.97.1.2 heterodimer alpha,beta, 1 * 100400 + 1 * 31300, mass spectrometry Pelobacter acidigallici

Cofactor

EC Number Cofactor Comment Organism Structure
1.97.1.2 molybdenum cofactor two molecules of molybdenum cofactor for each heterodimer, molybdenum is bound by a molybdopterin guanine dinucleotide Pelobacter acidigallici