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Literature summary extracted from

  • Kachroo, P.; Shanklin, J.; Shah, J.; Whittle, E.J.; Klessig, D.F.
    A fatty acid desaturase modulates the activation of defense signaling pathways in plants (2001), Proc. Natl. Acad. Sci. USA, 98, 9448-9453.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.19.1 agriculture disease resistance Arabidopsis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.1 expression in Escherichia coli, transfection of Arabidopsis by Agrobacterium tumefaciens clones Arabidopsis sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.1 L146F point mutation in ssi2 gene, causes reduced desaturase activity Arabidopsis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 stearoyl-acyl-carrier protein + AH2 + O2 Arabidopsis sp. key regulator of fatty acid desaturation of carbons 9 and 10 oleoyl-acyl-carrier protein + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Arabidopsis sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.1
-
 Arabidopsis sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.1 stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O SSI2 gene encodes a member of a family of soluble fatty acid desaturases Arabidopsis sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.1 leaf
-
 Arabidopsis sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.19.1 0.8
-
-
 Arabidopsis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 additional information preference for 18-carbon versus 16-carbon chain length fatty acids Arabidopsis sp. ?
-
 ?
1.14.19.1 stearoyl-acyl-carrier protein + AH2 + O2
-
 Arabidopsis sp. oleoyl-acyl-carrier protein + A + H2O
-
 ?
1.14.19.1 stearoyl-acyl-carrier protein + AH2 + O2 key regulator of fatty acid desaturation of carbons 9 and 10 Arabidopsis sp. oleoyl-acyl-carrier protein + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + AH2 + O2
-
 Arabidopsis sp. oleoyl-CoA + A + H2O
-
 ?