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Literature summary extracted from

  • Foot, M.; Jeffcoat, R.; Russell, N.
    Some properties, including the substrate in vivo, of the DELTA9-desaturase in Micrococcus cryophilus (1983), Biochem. J., 209, 345-353.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.1 azide at high concentrations Psychrobacter urativorans
1.14.19.1 CN- at high concentrations Psychrobacter urativorans
1.14.19.1 glutathione reduced, not marked Psychrobacter urativorans
1.14.19.1 L-cysteine not marked Psychrobacter urativorans
1.14.19.1 o-phenanthroline partially inhibitory Psychrobacter urativorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 membrane bound Psychrobacter urativorans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.19.1 Fe2+ 20-25% stimulation Psychrobacter urativorans
1.14.19.1 additional information no special ion requirement Psychrobacter urativorans
1.14.19.1 Zn2+ 20-25% stimulation Psychrobacter urativorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 additional information Psychrobacter urativorans biosynthesis of unsaturated fatty acids. Possible substrates: acyl-CoA, acyl-acyl-carrier and acyl chains of phospholipids. The substrate in vivo is saturated phospholipid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Psychrobacter urativorans
-
 ATCC 15174
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.1 stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O incorporation of fatty acid into phospholipid. Enzyme may contain an essential thiol group. Endogenous saturated phospholipid is desaturated rapidly in all experiments in contrast with exogenous saturated phospholipid, which generally is not desaturated Psychrobacter urativorans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 additional information not acyl-CoA as substrate but phospholipid Psychrobacter urativorans ?
-
 ?
1.14.19.1 additional information biosynthesis of unsaturated fatty acids. Possible substrates: acyl-CoA, acyl-acyl-carrier and acyl chains of phospholipids. The substrate in vivo is saturated phospholipid Psychrobacter urativorans ?
-
 ?
1.14.19.1 stearate + AH2 + O2
-
 Psychrobacter urativorans oleate + A + 2 H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.1 21
-
 assay at Psychrobacter urativorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.1 8.7
-
 but assay at pH 7.4, to standardize the experiments in vitro with those carried out with intact bacteria Psychrobacter urativorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 additional information requires some other form of reduced cofactor than a reduced nicotinamide nucleotide, no special cofactor requirement Psychrobacter urativorans