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Literature summary extracted from

  • Strittmatter, P.; Spatz, L.; Corcoran, D.; Rogers, M.J.; Setlow, B.; Redline, R.
    Purification and properties of rat liver microsomal stearyl coenzyme A desaturase (1974), Proc. Natl. Acad. Sci. USA, 71, 4565-4569.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.19.1 cytochrome b5 reductase NADH-specific, required, EC 1.6.2.2 Rattus norvegicus

General Stability

EC Number General Stability Organism
1.14.19.1 rapid isolation of enzyme and storage at -70°C is required Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.1 CN- complete and reversible inhibition at 0.5 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.19.1 Fe2+ contains one atom of non-heme iron per enzyme molecule, catalytic function Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.1 53000
-
 x * 53000, disc-gel electrophoresis Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 stearoyl-CoA + AH2 + O2 Rattus norvegicus
-
 oleoyl-CoA + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + AH2 + O2 Rattus norvegicus Long-Evans
-
 oleoyl-CoA + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Rattus norvegicus
-
 Long-Evans
-
1.14.19.1 Rattus norvegicus Long-Evans
-
 Long-Evans
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.19.1 phospholipoprotein tightly bound phospholipid is removed during purification Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.1 preparation of 11 fractions, ion-exchange, gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.19.1 0.24 0.36
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.14.19.1 enzyme cannot be stored overnight without loss of 20-50% of activity before fraction 5 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 stearoyl-CoA + AH2 + O2
-
 Rattus norvegicus oleoyl-CoA + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + AH2 + O2
-
 Rattus norvegicus Long-Evans oleoyl-CoA + A + H2O
-
 ?
1.14.19.1 stearoyl-CoA + NADH + O2 terminal component in the enzyme system Rattus norvegicus oleoyl-CoA + NAD+ + H2O
-
 ?
1.14.19.1 stearoyl-CoA + NADH + O2 terminal component in the enzyme system Rattus norvegicus Long-Evans oleoyl-CoA + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.1 ? x * 53000, disc-gel electrophoresis Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.1 25
-
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.19.1 32
-
 30 min, 70-80% of activity Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.19.1 0.35
-
 stearoyl-CoA 25°C Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 cytochrome b5 required Rattus norvegicus
1.14.19.1 additional information phospholipid is required Rattus norvegicus
1.14.19.1 NADH dependent Rattus norvegicus