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Literature summary extracted from

  • Vial, M.V.; Oelckers, K.B.; Rojas, M.C.; Simpfendrfer, R.W.
    Purification, partial kinetic characterization and reactive sulfhydryl groups of the phosphoenolpyruvate carboxykinase from Perumytilus purpuratus adductor muscle (1995), Comp. Biochem. Physiol. B, 112, 451-460.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.32 5,5'-dithiobis(2-nitrobenzoate)
-
 Perumytilus purpuratus
4.1.1.32 ITP
-
 Perumytilus purpuratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 0.045
-
 IDP pH 7, in presence of 2.3 mM Mn2+ Perumytilus purpuratus
4.1.1.32 0.55
-
 phosphoenolpyruvate pH 7, in presence of 2.3 mM Mn2+ Perumytilus purpuratus
4.1.1.32 2.4
-
 CO2 pH 7, in presence of 2.3 mM Mn2+ Perumytilus purpuratus
4.1.1.32 2.4
-
 CO2 CO2 in form of HCO3- Perumytilus purpuratus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Co2+ divalent metal ion required, decreasing order of effectiveness: Co2+, Mn2+, Zn2+, Mg2+ Perumytilus purpuratus
4.1.1.32 Mg2+ divalent metal ion required, decreasing order of effectiveness: Co2+, Mn2+, Zn2+, Mg2+ Perumytilus purpuratus
4.1.1.32 Mn2+ divalent metal ion required, decreasing order of effectiveness: Co2+, Mn2+, Zn2+, Mg2+ Perumytilus purpuratus
4.1.1.32 Zn2+ divalent metal ion required, decreasing order of effectiveness: Co2+, Mn2+, Zn2+, Mg2+ Perumytilus purpuratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.32 74000
-
 1 * 74000, SDS-PAGE Perumytilus purpuratus
4.1.1.32 85000
-
 gel filtration Perumytilus purpuratus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Perumytilus purpuratus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.32
-
 Perumytilus purpuratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.32 adductor
-
 Perumytilus purpuratus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.32 13
-
-
 Perumytilus purpuratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GDP + phosphoenolpyruvate + CO2
-
 Perumytilus purpuratus GTP + oxaloacetate
-
 r
4.1.1.32 GTP + oxaloacetate
-
 Perumytilus purpuratus GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 IDP + phosphoenolpyruvate + CO2
-
 Perumytilus purpuratus ITP + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.32 monomer 1 * 74000, SDS-PAGE Perumytilus purpuratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.32 6.7
-
-
 Perumytilus purpuratus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.32 6 8 pH 6: about 50% of maximal activity, pH 8: about 25% of maximal activity Perumytilus purpuratus