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Literature summary extracted from

  • Colombo, G.; Carlson, G.M.; Lardy, H.A.
    Phosphoenolpyruvate carboxykinase (guanosine triphosphate) from rat liver cytosol. Separation of homogenous forms of the enzyme with high and low activity by chromatography on agarose-hexane-guanosine triphosphate (1978), Biochemistry, 17, 5321-5329.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.32 dithiothreitol 5 mM, 19% inhibition Rattus norvegicus
4.1.1.32 EDTA 0.5 mM, 86% inhibition Rattus norvegicus
4.1.1.32 FeCl2 1.5 mM FeCl2 causes rapid inactivation. 0.05 mM FeCl2 and 0.0044 mg/ml ferroactivator, enhance to 3.1times the unstimulated rate Rattus norvegicus
4.1.1.32 MgCl2 1.5 mM, 62% inhibition Rattus norvegicus
4.1.1.32 Mn2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 0.0075
-
 GDP
-
 Rattus norvegicus
4.1.1.32 0.0211
-
 GTP
-
 Rattus norvegicus
4.1.1.32 0.026
-
 oxaloacetate
-
 Rattus norvegicus
4.1.1.32 0.048
-
 phosphoenolpyruvate
-
 Rattus norvegicus
4.1.1.32 0.0715
-
 IDP
-
 Rattus norvegicus
4.1.1.32 0.279
-
 ITP
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Fe2+ 0.05 mM FeCl2 and 0.0044 mg/ml ferroactivator, enhance to 3.1times the unstimulated rate. 1.5 mM FeCl2 causes rapid inactivation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.32 71500
-
 1 * 71500, SDS-PAGE Rattus norvegicus
4.1.1.32 74000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.32 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.32 16.7
-
 carboxylation of phosphoenolpyruvate Rattus norvegicus
4.1.1.32 19.9
-
 decarboxylation of oxaloacetate Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
4.1.1.32 4°C, in absence of metal ions, under N2, protein concentration 0.5 mg/ml or higher, stable for at least 14 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GDP + phosphoenolpyruvate + CO2
-
 Rattus norvegicus GTP + oxaloacetate
-
 ?
4.1.1.32 GTP + oxaloacetate
-
 Rattus norvegicus GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 IDP + phosphoenolpyruvate + CO2
-
 Rattus norvegicus ITP + oxaloacetate
-
 ?
4.1.1.32 ITP + oxaloacetate
-
 Rattus norvegicus IDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 oxaloacetate + ? ir Rattus norvegicus pyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.32 monomer 1 * 71500, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.32 22
-
 protein concentration 0.057 mg/ml, glutathione concentration 0.2 mM, stable for at least 3 h Rattus norvegicus