EC Number | General Stability | Organism |
---|---|---|
6.1.1.4 | 20-30% loss of activity after 1 cycle of freezing and thawing | Euglena gracilis |
6.1.1.4 | stability is lower with decreasing protein concentration | Euglena gracilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.1.1.4 | chloroplast | - |
Euglena gracilis | 9507 | - |
6.1.1.4 | cytoplasm | - |
Euglena gracilis | 5737 | - |
6.1.1.9 | cytoplasm | - |
Euglena gracilis | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.4 | 100000 | - |
1 * 100000, chloroplastic enzyme, SDS-PAGE | Euglena gracilis |
6.1.1.4 | 116000 | - |
1 * 116000, cytoplasmic enzyme, SDS-PAGE | Euglena gracilis |
6.1.1.9 | 126000 | - |
1 * 126000, urea-SDS-PAGE | Euglena gracilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.4 | Euglena gracilis | - |
- |
- |
6.1.1.9 | Euglena gracilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.4 | - |
Euglena gracilis |
6.1.1.9 | - |
Euglena gracilis |
EC Number | Storage Stability | Organism |
---|---|---|
6.1.1.4 | 4°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 0.002 mM L-leucine, 100 mM NaCl, 15% v/v 1,2-propanediol, stable for several weeks | Euglena gracilis |
6.1.1.9 | 4°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 0.002 mM L-valine, 100 mM NaCl, 15% v/v 1,2-propanediol, stable for several weeks | Euglena gracilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.4 | ATP + L-leucine + tRNALeu | - |
Euglena gracilis | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
6.1.1.9 | ATP + L-valine + tRNAVal | - |
Euglena gracilis | AMP + diphosphate + L-valyl-tRNAVal | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.4 | monomer | 1 * 116000, cytoplasmic enzyme, SDS-PAGE | Euglena gracilis |
6.1.1.4 | monomer | 1 * 100000, chloroplastic enzyme, SDS-PAGE | Euglena gracilis |
6.1.1.9 | monomer | 1 * 126000, urea-SDS-PAGE | Euglena gracilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.9 | 40 | - |
5 min, chloroplastic enzyme loses 30% of its activity, mitochondrial enzyme 75% | Euglena gracilis |