EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.31 | acetoacetyl-CoA | activates | Starkeya novella | |
4.1.1.31 | butyryl-CoA | activates | Starkeya novella | |
4.1.1.31 | heptanoyl-CoA | activates | Starkeya novella | |
4.1.1.31 | hexanoyl-CoA | activates | Starkeya novella | |
4.1.1.31 | propionyl-CoA | activates | Starkeya novella | |
4.1.1.31 | succinyl-CoA | activates | Starkeya novella | |
4.1.1.31 | valeryl-CoA | activates | Starkeya novella |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.31 | additional information | - |
additional information | - |
Starkeya novella | |
4.1.1.31 | 0.11 | - |
HCO3- | - |
Starkeya novella | |
4.1.1.31 | 0.64 | - |
phosphoenolpyruvate | - |
Starkeya novella |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.31 | Cd2+ | 28% of the activation with Mg2+ | Starkeya novella | |
4.1.1.31 | Co2+ | 30% of the activation with Mg2+ | Starkeya novella | |
4.1.1.31 | Mg2+ | divalent cation required, Mg2+ is the best activator | Starkeya novella | |
4.1.1.31 | Mn2+ | 68% of the activation with Mg2+. At suboptimal concentrations of phosphoenolpyruvate and HCO3-, Mn2+ is more effective than Mg2+ | Starkeya novella |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.31 | 95000 | - |
4 * 95000, SDS-PAGE | Starkeya novella |
4.1.1.31 | 360000 | - |
gel filtration | Starkeya novella |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.31 | Phosphoenolpyruvate + CO2 | Starkeya novella | function is probably anaplerotic | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.31 | Starkeya novella | - |
strain ATCC 90893 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.31 | - |
Starkeya novella |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.31 | 51.09 | - |
- |
Starkeya novella |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.1.31 | -20°C, 0.04 M Tris/HCl buffer, pH 7.8, 2.0 mM dithiothreitol, 2.0 mM MgCl2, 1.0 mM EDTA, 20% glycerol, 1.0 mM Pro, stable for at least 4 weeks | Starkeya novella |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.31 | phosphoenolpyruvate + CO2 | - |
Starkeya novella | phosphate + oxaloacetate | - |
? | |
4.1.1.31 | Phosphoenolpyruvate + CO2 | function is probably anaplerotic | Starkeya novella | ? | - |
? | |
4.1.1.31 | phosphoenolpyruvate + HCO3- | - |
Starkeya novella | phosphate + oxaloacetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.31 | tetramer | 4 * 95000, SDS-PAGE | Starkeya novella |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.31 | 8 | - |
- |
Starkeya novella |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.31 | acetyl-CoA | activates | Starkeya novella |