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Literature summary extracted from

  • Owttrim, G.W.; Colman, B.
    Purification and characterization of phosphoenolpyruvate carboxylase from a cyanobacterium (1986), J. Bacteriol., 168, 207-212.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 3-Phosphoglyceric acid
-
 Coccochloris peniocystis
4.1.1.31 pyruvate activates Coccochloris peniocystis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 2-oxoglutarate
-
 Coccochloris peniocystis
4.1.1.31 alpha-Hydroxy-2-pyridylmethanesulfonate
-
 Coccochloris peniocystis
4.1.1.31 ATP
-
 Coccochloris peniocystis
4.1.1.31 citrate
-
 Coccochloris peniocystis
4.1.1.31 isocitrate
-
 Coccochloris peniocystis
4.1.1.31 Maleate
-
 Coccochloris peniocystis
4.1.1.31 malonate
-
 Coccochloris peniocystis
4.1.1.31 oxaloacetate
-
 Coccochloris peniocystis
4.1.1.31 phosphate
-
 Coccochloris peniocystis
4.1.1.31 Picolinic acid
-
 Coccochloris peniocystis
4.1.1.31 quinolinic acid
-
 Coccochloris peniocystis
4.1.1.31 SO42-
-
 Coccochloris peniocystis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.6
-
 phosphoenolpyruvate
-
 Coccochloris peniocystis
4.1.1.31 0.8
-
 HCO3-
-
 Coccochloris peniocystis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Co2+ 60% of the activation with Mg2+ Coccochloris peniocystis
4.1.1.31 Mg2+ activates Coccochloris peniocystis
4.1.1.31 Mg2+ Km: 0.27 mM Coccochloris peniocystis
4.1.1.31 Mn2+ 80% of the activation with Mg2+ Coccochloris peniocystis
4.1.1.31 NaCl activation between 25 and 200 mM to a maximum of 14% a at 75 mM Coccochloris peniocystis
4.1.1.31 Zn2+ 60% of the activation with Mg2+ Coccochloris peniocystis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 560000
-
 gel filtration Coccochloris peniocystis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Coccochloris peniocystis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Coccochloris peniocystis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 8.87
-
-
 Coccochloris peniocystis

Storage Stability

EC Number Storage Stability Organism
4.1.1.31 -80°C, concentrated, undesalted solution containing 33% v/v glycerol, stable for at least 6 weeks Coccochloris peniocystis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Coccochloris peniocystis phosphate + oxaloacetate
-
 ?
4.1.1.31 phosphoenolpyruvate + HCO3-
-
 Coccochloris peniocystis phosphate + oxaloacetate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.31 40
-
-
 Coccochloris peniocystis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 8
-
-
 Coccochloris peniocystis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.31 NADPH activates Coccochloris peniocystis