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Literature summary extracted from

  • Schwitzguebel, J.P.; Ettlinger, L.
    Phosphoenolpyruvate carboxylase from Acetobacter aceti (1979), Arch. Microbiol., 122, 109-115.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 propionyl-CoA absolute requirement for acetyl-CoA or propionyl-CoA Acetobacter aceti

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 2-oxoglutarate
-
 Acetobacter aceti
4.1.1.31 Asp
-
 Acetobacter aceti
4.1.1.31 citrate
-
 Acetobacter aceti
4.1.1.31 Co2+ maximal activity at 1 mM Co2+, strong decrease of activity above 4 mM Acetobacter aceti
4.1.1.31 fumarate
-
 Acetobacter aceti
4.1.1.31 Glu
-
 Acetobacter aceti
4.1.1.31 Mn2+ maximal activity at 0.5 mM Mn2+, strong decrease of activity above 2 mM Acetobacter aceti
4.1.1.31 p-hydroxymercuribenzoate glutathione protects Acetobacter aceti
4.1.1.31 PCMB glutathione protects Acetobacter aceti
4.1.1.31 succinate
-
 Acetobacter aceti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.49
-
 phosphoenolpyruvate
-
 Acetobacter aceti
4.1.1.31 3
-
 HCO3-
-
 Acetobacter aceti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Co2+ divalent cation required, Mn2+, Co2+ and Mg2+. Co2+ is less effective than Mg2+ or Mn2+ Acetobacter aceti
4.1.1.31 Co2+ maximal activity at 1 mM Co2+, strong decrease of activity above 4 mM Acetobacter aceti
4.1.1.31 Co2+ Km: 0.26 mM Acetobacter aceti
4.1.1.31 Mg2+ Km: 0.77 mM Acetobacter aceti
4.1.1.31 Mg2+ divalent cation required, Mn2+, Co2+ and Mg2+ Acetobacter aceti
4.1.1.31 Mn2+ divalent cation required, Mn2+, Co2+ and Mg2+ Acetobacter aceti
4.1.1.31 Mn2+ Km: 0.12 mM Acetobacter aceti
4.1.1.31 Mn2+ maximal activity at 0.5 mM Mn2+, strong decrease of activity above 2 mM Acetobacter aceti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 380000 400000 gel filtration Acetobacter aceti

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Acetobacter aceti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Acetobacter aceti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 15.9
-
-
 Acetobacter aceti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Acetobacter aceti phosphate + oxaloacetate
-
 ?
4.1.1.31 phosphoenolpyruvate + HCO3-
-
 Acetobacter aceti phosphate + oxaloacetate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.31 55
-
-
 Acetobacter aceti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 60 65 rapid inactivation Acetobacter aceti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 7.5
-
-
 Acetobacter aceti

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.31 6.5 9 about 80% of maximal activity at pH 6.5 and at pH 9.0 Acetobacter aceti

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.31 acetyl-CoA absolute requirement for acetyl-CoA or propionyl-CoA Acetobacter aceti