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Literature summary extracted from

  • Chirikjian, J.G.; Kanagalingam, K.; Lau, E.; Fresco, J.R.
    Purification and properties of leucyl-tRNA synthetase from bakers' yeast (1973), J. Biol. Chem., 248, 1074-1079.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.4 DTT stabilizes Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.4 Cd2+ in presence of 0.1 mM Mg2+ Saccharomyces cerevisiae
6.1.1.4 DTNB
-
 Saccharomyces cerevisiae
6.1.1.4 DTT
-
 Saccharomyces cerevisiae
6.1.1.4 p-chloromercuribenzoate
-
 Saccharomyces cerevisiae
6.1.1.4 Zn2+ in presence of 0.1 mM Mg2+ Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.0004
-
 tRNALeu
-
 Saccharomyces cerevisiae
6.1.1.4 0.02
-
 L-Leu
-
 Saccharomyces cerevisiae
6.1.1.4 0.11
-
 ATP
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 Ba2+ Mg2+, Mn2+, Ca2+ and Ba2+ similarly effective, optimal concentration: 0.1 mM Saccharomyces cerevisiae
6.1.1.4 Ca2+ Mg2+, Mn2+, Ca2+ and Ba2+ similarly effective, optimal concentration: 0.1 mM Saccharomyces cerevisiae
6.1.1.4 Mg2+ Mg2+, Mn2+, Ca2+ and Ba2+ similarly effective, optimal concentration: 0.1 mM Saccharomyces cerevisiae
6.1.1.4 Mn2+ Mg2+, Mn2+, Ca2+ and Ba2+ similarly effective, optimal concentration: 0.1 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 119000 120000 equilibrium sedimentation method Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.4 1.804
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
6.1.1.4 -90°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Saccharomyces cerevisiae AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 additional information no substrates: dATP, GTP, dGTP Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 ? x * 55-60000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 33 37
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.4 45
-
 rapid inactivation Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 7.5
-
 in cacodylate(K+) buffer or HEPES buffer Saccharomyces cerevisiae