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Literature summary extracted from

  • Broquist, H.P.
    Saccharopine dehydrogenase (1971), Methods Enzymol., 17B, 124-129.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.5.1.7 2-mercaptoethanol, 1 mM, stabilizes Saccharomyces cerevisiae
1.5.1.7 high salt concentration stabilizes Saccharomyces cerevisiae
1.5.1.7 KCl, above 0.1 M stablizes Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.7 p-hydroxymercuribenzoate inhibition counteracted by 2-mercaptoethanol or glutathione Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.7 0.44
-
2-oxoglutarate
-
Saccharomyces cerevisiae
1.5.1.7 0.46
-
NADH
-
Saccharomyces cerevisiae
1.5.1.7 12
-
L-lysine
-
Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.7 49000
-
sucrose density gradient centrifugation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.7 Saccharomyces cerevisiae
-
baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.7
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.7 additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O high specificity with respect to coenzyme and substrate Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
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Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.7 25
-
assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.7 7
-
L-lysine + 2-oxoglutarate + NADH Saccharomyces cerevisiae
1.5.1.7 10
-
or higher, saccharopine + NAD+ + H2O Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.7 additional information 5% of NADH reaction Saccharomyces cerevisiae
1.5.1.7 NAD+
-
Saccharomyces cerevisiae