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Literature summary extracted from
- Comparison of L-aspartate 4-carboxy-lyases of Cunninghammela elegans and Penicillium citrinum (1994), Microbiol. Res., 149, 253-157.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.12 | 2-oxoglutarate | activates | Cunninghamella elegans |  |
| 4.1.1.12 | 2-oxoglutarate | activates | Penicillium citrinum | |
| 4.1.1.12 | oxalacetate | - |
Cunninghamella elegans | |
| 4.1.1.12 | oxalacetate | - |
Penicillium citrinum | |
| 4.1.1.12 | pyruvate | activates | Cunninghamella elegans | |
| 4.1.1.12 | pyruvate | activates | Penicillium citrinum | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.12 | Cu2+ | - |
Cunninghamella elegans | |
| 4.1.1.12 | Cu2+ | - |
Penicillium citrinum | |
| 4.1.1.12 | EDTA | - |
Cunninghamella elegans | |
| 4.1.1.12 | EDTA | - |
Penicillium citrinum | |
| 4.1.1.12 | iodoacetate | - |
Cunninghamella elegans | |
| 4.1.1.12 | iodoacetate | - |
Penicillium citrinum | |
| 4.1.1.12 | Zn2+ | - |
Cunninghamella elegans | |
| 4.1.1.12 | Zn2+ | - |
Penicillium citrinum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.12 | 25 | - |
L-Asp | - |
Cunninghamella elegans | |
| 4.1.1.12 | 27 | - |
L-Asp | - |
Penicillium citrinum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.12 | Co2+ | stimulates | Cunninghamella elegans | |
| 4.1.1.12 | Co2+ | stimulates | Penicillium citrinum | |
| 4.1.1.12 | Fe2+ | stimulates | Cunninghamella elegans | |
| 4.1.1.12 | Fe2+ | stimulates | Penicillium citrinum | |
| 4.1.1.12 | Mn2+ | stimulates | Cunninghamella elegans | |
| 4.1.1.12 | Mn2+ | stimulates | Penicillium citrinum | |
| 4.1.1.12 | Ni2+ | stimulates | Cunninghamella elegans | |
| 4.1.1.12 | Ni2+ | stimulates | Penicillium citrinum | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.12 | Cunninghamella elegans | - |
- |
- |
| 4.1.1.12 | Penicillium citrinum | - |
- |
- |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.1.12 | -15°C, 0.05 M acetate buffer, pH 5.2, 52% loss of activity after 6 days, complete loss of activity after 16 days | Cunninghamella elegans |
| 4.1.1.12 | -15°C, 0.05 M acetate buffer, pH 5.2, 59% loss of activity after 6 days, complete loss of activity after 12 days | Penicillium citrinum |
| 4.1.1.12 | 4°C, 0.05 M acetate buffer, pH 5.2, rapid loss of activity | Cunninghamella elegans |
| 4.1.1.12 | 4°C, 0.05 M acetate buffer, pH 5.2, rapid loss of activity | Penicillium citrinum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.12 | L-Asp | highly specific for L-Asp | Cunninghamella elegans | L-Ala + CO2 | - |
? | |
| 4.1.1.12 | L-Asp | highly specific for L-Asp | Penicillium citrinum | L-Ala + CO2 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.12 | 40 | - |
- |
Cunninghamella elegans |
| 4.1.1.12 | 40 | - |
- |
Penicillium citrinum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.12 | 30 | 50 | 30°C: about 65% of maximal activity, 50°C: about 50% of maximal activity | Cunninghamella elegans |
| 4.1.1.12 | 30 | 50 | 30°C: about 60% of maximal activity, 50°C: about 35% of maximal activity | Penicillium citrinum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.12 | 50 | - |
30% loss of activity after 15 min, 75% loss of activity after 40 min | Cunninghamella elegans |
| 4.1.1.12 | 50 | - |
37% loss of activity after 15 min, 85% loss of activity after 40 min | Penicillium citrinum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.12 | 5.5 | - |
- |
Cunninghamella elegans |
| 4.1.1.12 | 5.5 | - |
- |
Penicillium citrinum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.12 | 4 | 8 | pH 4.0: about 65% of maximal activity, pH 8.0: about 40% of maximal activity | Cunninghamella elegans |
| 4.1.1.12 | 4 | 8 | pH 4.0: about 60% of maximal activity, pH 8.0: about 35% of maximal activity | Penicillium citrinum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.12 | pyridoxal 5'-phosphate | activates | Cunninghamella elegans | |
| 4.1.1.12 | pyridoxal 5'-phosphate | activates | Penicillium citrinum | |
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