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Literature summary extracted from

  • D'Annibale, A.; Celletti, D.; Felici, M.; Di Mattia, E.; Giovannozzi-Sermanni, G.
    Substrate specificity of laccase from Lentinus edodes (1996), Acta Biotechnol., 16, 257-270.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 azide 100% inhibition at 0.1 mM Lentinula edodes
1.10.3.2 diethyldithiocarbamate 35% inhibition at 1 mM Lentinula edodes
1.10.3.2 sodium dodecylsulfate 50% inhibition at 1 mM Lentinula edodes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.03
-
 pyrogallol
-
 Lentinula edodes
1.10.3.2 0.043
-
 caffeic acid
-
 Lentinula edodes
1.10.3.2 0.08
-
 2,6-dimethoxyphenol
-
 Lentinula edodes
1.10.3.2 0.09
-
 guaiacol
-
 Lentinula edodes
1.10.3.2 0.11
-
 hydroquinone
-
 Lentinula edodes
1.10.3.2 0.11
-
 Sinapic acid
-
 Lentinula edodes
1.10.3.2 0.13
-
 gallic acid
-
 Lentinula edodes
1.10.3.2 0.175
-
 4-methyl-catechol
-
 Lentinula edodes
1.10.3.2 0.22
-
 catechol
-
 Lentinula edodes
1.10.3.2 0.24
-
 ferulic acid
-
 Lentinula edodes
1.10.3.2 0.24
-
 p-coumaric acid
-
 Lentinula edodes
1.10.3.2 0.33
-
 p-methoxy-phenol
-
 Lentinula edodes
1.10.3.2 0.65
-
 levo-dopa
-
 Lentinula edodes
1.10.3.2 1.35
-
 o-Chlorophenol
-
 Lentinula edodes
1.10.3.2 1.74
-
 p-chlorophenol
-
 Lentinula edodes
1.10.3.2 2.2
-
 p-Cresol
-
 Lentinula edodes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper
-
 Lentinula edodes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 37000
-
 2 * 37000, SDS-PAGE Lentinula edodes
1.10.3.2 74000
-
 gel filtration Lentinula edodes

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Lentinula edodes
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein sugar content 7.5% Lentinula edodes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2 catechol + O2
-
 Lentinula edodes 2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 4-methylcatechol + O2
-
 Lentinula edodes 6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
 ?
1.10.3.2 caffeic acid + O2
-
 Lentinula edodes ?
-
 ?
1.10.3.2 dopa + O2
-
 Lentinula edodes ?
-
 ?
1.10.3.2 ferulic acid + O2
-
 Lentinula edodes 3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
 ?
1.10.3.2 gallic acid + O2
-
 Lentinula edodes ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Lentinula edodes 6-methoxycyclohexa-2,4-dienone + H2O
-
 ?
1.10.3.2 o-chlorophenol + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 p-chlorophenol + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 p-coumaric acid + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 p-cresol + O2
-
 Lentinula edodes 6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
 ?
1.10.3.2 p-methoxyphenol + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 phenol + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 pyrogallol + O2
-
 Lentinula edodes ?
-
 ?
1.10.3.2 quinol + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 sinapic acid + O2
-
 Lentinula edodes 3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 dimer 2 * 37000, SDS-PAGE Lentinula edodes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
-
 Lentinula edodes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 stable for at least 12 h Lentinula edodes
1.10.3.2 50
-
 half life 130 min Lentinula edodes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4 4.2
-
 Lentinula edodes