BRENDA - Enzyme Database

Purification and properties of polyphenol oxidase from Chaetomium thermophile, a thermophilic fungus

Ishigami, T.; Yamada, Y.; J. Gen. Appl. Microbiol. 32, 293-301 (1986)
No PubMed abstract available

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.10.3.2
extracellular
-
Chaetomium thermophilum
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.2
96000
110000
gel filtration
Chaetomium thermophilum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.2
Chaetomium thermophilum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.10.3.2
-
Chaetomium thermophilum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.2
culture filtrate
-
Chaetomium thermophilum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.2
additional information
-
depending on isozyme, substrate and pH
Chaetomium thermophilum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.2
D-catechin + O2
-
396347
Chaetomium thermophilum
?
-
-
-
-
1.10.3.2
N,N-dimethyl-p-phenylenediamine + O2
-
396347
Chaetomium thermophilum
?
-
-
-
?
1.10.3.2
quinol + O2
-
396347
Chaetomium thermophilum
? + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.2
55
-
-
Chaetomium thermophilum
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.10.3.2
45
60
-
Chaetomium thermophilum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.10.3.2
55
-
stable below
Chaetomium thermophilum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.2
5
-
-
Chaetomium thermophilum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.10.3.2
4.5
5.5
-
Chaetomium thermophilum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.10.3.2
4
11
-
Chaetomium thermophilum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.10.3.2
extracellular
-
Chaetomium thermophilum
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.2
96000
110000
gel filtration
Chaetomium thermophilum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.10.3.2
-
Chaetomium thermophilum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.2
culture filtrate
-
Chaetomium thermophilum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.2
additional information
-
depending on isozyme, substrate and pH
Chaetomium thermophilum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.2
D-catechin + O2
-
396347
Chaetomium thermophilum
?
-
-
-
-
1.10.3.2
N,N-dimethyl-p-phenylenediamine + O2
-
396347
Chaetomium thermophilum
?
-
-
-
?
1.10.3.2
quinol + O2
-
396347
Chaetomium thermophilum
? + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.2
55
-
-
Chaetomium thermophilum
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.10.3.2
45
60
-
Chaetomium thermophilum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.10.3.2
55
-
stable below
Chaetomium thermophilum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.2
5
-
-
Chaetomium thermophilum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.10.3.2
4.5
5.5
-
Chaetomium thermophilum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.10.3.2
4
11
-
Chaetomium thermophilum