EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.11.9 | - |
Pseudomonas putida |
EC Number | General Stability | Organism |
---|---|---|
1.13.11.9 | combination of dithiothreitol and FeSO4 is detrimental during long-term incubation at 0°C | Pseudomonas putida |
1.13.11.9 | dithiothreitol stabilizes | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.9 | 1,10-phenanthroline | - |
Pseudomonas putida | |
1.13.11.9 | 2,2'-dipyridyl | - |
Pseudomonas putida | |
1.13.11.9 | EDTA | - |
Pseudomonas putida | |
1.13.11.9 | H2O2 | - |
Pseudomonas putida | |
1.13.11.9 | iodoacetamide | - |
Pseudomonas putida | |
1.13.11.9 | KCN | - |
Pseudomonas putida | |
1.13.11.9 | N-ethylmaleimide | - |
Pseudomonas putida | |
1.13.11.9 | p-chloromercuribenzoate | - |
Pseudomonas putida | |
1.13.11.9 | Tiron | i.e. 4,5-dihydroxy-1,3-benzene disulfonic acid | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.9 | Iron | Fe2+ requirement | Pseudomonas putida | |
1.13.11.9 | Iron | enzyme contains loosly bound Fe3+ | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.9 | 39500 | - |
x * 39500, SDS-PAGE | Pseudomonas putida |
1.13.11.9 | 242000 | - |
sucrose density gradient centrifugation (+ dithiothreitol) | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.9 | 2,5-dihydroxypyridine + O2 + H2O | Pseudomonas putida | nicotinic acid catabolism | maleamate + formate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.9 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.9 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.9 | 38.3 | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.13.11.9 | -40°C, 3 months, 20% loss of activity | Pseudomonas putida |
1.13.11.9 | 0°C, 20 mM sodium phosphate, pH 7.5, half-life: 2-3 days, addition of dithiothreitol extends half-life to about 2 weeks | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.9 | 2,5-dihydroxypyridine + O2 + H2O | strictly specific for 2,5-dihydroxypyridine | Pseudomonas putida | maleamate + formate | - |
? | |
1.13.11.9 | 2,5-dihydroxypyridine + O2 + H2O | nicotinic acid catabolism | Pseudomonas putida | maleamate + formate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.9 | ? | x * 39500, SDS-PAGE | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.9 | 8 | - |
- |
Pseudomonas putida |