EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.5 | ascorbate | required for maximal activity at pH 6 | Pseudomonas fluorescens | |
1.13.11.5 | ascorbate | required for maximal activity at pH 7 | Bos taurus | |
1.13.11.5 | glutathione | required for maximal activity at pH 6 and 5.4 | Pseudomonas fluorescens | |
1.13.11.5 | glutathione | reduced glutathione is required for maximal activity at pH 7 | Bos taurus | |
1.13.11.5 | additional information | pH 7.0: a combination of ascorbate, reduced glutathione and ferrous iron is required for activation, pH 5.3: ferrous iron alone fully activates | Bos taurus | |
1.13.11.19 | hydroxylamine | cofactor-like compound | Equus caballus | |
1.13.11.19 | hydroxylamine | sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) | Equus caballus | |
1.13.11.19 | Se | cofactor-like compound | Equus caballus | |
1.13.11.19 | Se | sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) | Equus caballus | |
1.13.11.19 | Sulfide | cofactor-like compound | Equus caballus | |
1.13.11.19 | Sulfide | sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) | Equus caballus | |
1.13.11.19 | sulfur | cofactor-like compound | Equus caballus | |
1.13.11.19 | sulfur | sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) | Equus caballus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.11.5 | - |
Pseudomonas fluorescens |
1.13.11.9 | - |
Pseudomonas putida |
1.13.11.15 | - |
Pseudomonas putida |
EC Number | General Stability | Organism |
---|---|---|
1.13.11.5 | 10% acetone stabilizes | Bos taurus |
1.13.11.5 | unlike the mammalian enzyme, the bacterial enzyme is fairly stable on aging or during storage | Pseudomonas fluorescens |
1.13.11.9 | combination of dithiothreitol and FeSO4 is detrimental during long-term incubation at 0°C | Pseudomonas putida |
1.13.11.9 | dithiothreitol stabilizes | Pseudomonas putida |
1.13.11.15 | stabilization in presence of low concentrations of acetone or ethanol | Pseudomonas putida |
1.13.11.25 | acetone stabilizes | Rhodococcus equi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.5 | Lewisite | inhibits by competing with Fe2+ for a common enzymic binding site | Bos taurus | |
1.13.11.5 | Methylmercuric bromide | inhibits by competing with Fe2+ for a common enzymic binding site | Bos taurus | |
1.13.11.5 | p-chloromercuribenzoate | inhibits by competing with Fe2+ for a common enzymic binding site | Bos taurus | |
1.13.11.5 | p-chloromercuribenzoate | competitive inhibition with respect to Fe2+ but not to homogentisate | Pseudomonas fluorescens | |
1.13.11.9 | 1,10-phenanthroline | - |
Pseudomonas putida | |
1.13.11.9 | 2,2'-dipyridyl | - |
Pseudomonas putida | |
1.13.11.9 | EDTA | - |
Pseudomonas putida | |
1.13.11.9 | H2O2 | - |
Pseudomonas putida | |
1.13.11.9 | KCN | - |
Pseudomonas putida | |
1.13.11.9 | N-ethylmaleimide | - |
Pseudomonas putida | |
1.13.11.9 | p-chloromercuribenzoate | - |
Pseudomonas putida | |
1.13.11.19 | S | sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) | Equus caballus | |
1.13.11.19 | Se | sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) | Equus caballus | |
1.13.11.19 | Sulfide | sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine) | Equus caballus | |
1.13.11.25 | 2,2'-dipyridyl | noncompetitive inhibition | Rhodococcus equi | |
1.13.11.25 | 4-hydroxymercuribenzoate | 1 mM, 87% inhibition after 10 min | Rhodococcus equi | |
1.13.11.25 | 4-Isopropylcatechol | competitive vs. organic substrate, uncompetitive vs. O2 | Rhodococcus equi | |
1.13.11.25 | 8-hydroxyquinoline | 1 mM, 90% inhibition after 10 min, noncompetitive inhibition | Rhodococcus equi | |
1.13.11.25 | o-phenanthroline | 1 mM, 68% inhibition after 10 min, noncompetitive inhibition | Rhodococcus equi | |
1.13.11.25 | sodium iodoacetate | 1 mM, 70% inhibition after 10 min | Rhodococcus equi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.5 | 0.6 | - |
homogentisate | at pH 6 | Pseudomonas fluorescens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.13.11.5 | soluble | - |
Bos taurus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.5 | Fe2+ | ferrous mercaptans at the active center | Bos taurus | |
1.13.11.5 | Fe2+ | Fe2+ is required as cofactor | Pseudomonas fluorescens | |
1.13.11.5 | Fe2+ | ferrous ion is required for maximal activity at pH 7 and pH 5.3 | Bos taurus | |
1.13.11.15 | Iron | 4-5 gatom of iron per mol of enzyme, most of the iron is involved in the association of the subunits, at least 1 gatom of iron is at the active site | Pseudomonas putida | |
1.13.11.19 | Fe | a non-heme-iron protein, contains 1 g-atom of iron per mol of enzyme (based on a MW of 83000), nearly all of the iron is in the ferric state | Equus caballus | |
1.13.11.25 | Fe2+ | 1.13 gatom per mol of enzyme, stimulation of reaction | Rhodococcus equi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.5 | 380000 | - |
ultracentrifugal analysis | Pseudomonas fluorescens |
1.13.11.9 | 39500 | - |
x * 39500, SDS-PAGE | Pseudomonas putida |
1.13.11.9 | 242000 | - |
sucrose density gradient centrifugation (+ dithiothreitol) | Pseudomonas putida |
1.13.11.19 | 50000 | - |
2 * 50000 SDS-PAGE | Equus caballus |
1.13.11.19 | 100000 | - |
method not mentioned | Equus caballus |
1.13.11.25 | 286000 | - |
- |
Rhodococcus equi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.25 | 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 | Rhodococcus equi | - |
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.5 | Bos taurus | - |
- |
- |
1.13.11.5 | Pseudomonas fluorescens | - |
- |
- |
1.13.11.9 | Pseudomonas putida | - |
- |
- |
1.13.11.15 | Pseudomonas putida | - |
- |
- |
1.13.11.19 | Equus caballus | - |
- |
- |
1.13.11.25 | Rhodococcus equi | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.13.11.5 | Fe2+, organic mercurials and reducing agents protect the enzyme from irreversible aerobic oxidation | Bos taurus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.5 | partial purification | Bos taurus |
1.13.11.15 | - |
Pseudomonas putida |
1.13.11.19 | - |
Equus caballus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.13.11.25 | 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate | proximal extradiol cleavage | Rhodococcus equi |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.19 | kidney | - |
Equus caballus | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.13.11.19 | 0°C, 70% saturated ammonium sulfate, stable for months | Equus caballus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.5 | homogentisate + O2 | - |
Pseudomonas fluorescens | 4-maleylacetoacetate | - |
? | |
1.13.11.9 | 2,5-dihydroxypyridine + O2 + H2O | - |
Pseudomonas putida | maleamate + formate | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | - |
Pseudomonas putida | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.13.11.19 | cysteamine + O2 | - |
Equus caballus | hypotaurine | - |
? | |
1.13.11.25 | 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 | - |
Rhodococcus equi | 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate | - |
? | |
1.13.11.25 | 3-isopropylcatechol + O2 | - |
Rhodococcus equi | ? | - |
? | |
1.13.11.25 | 3-tert-butyl-5-methylcatechol + O2 | - |
Rhodococcus equi | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.9 | ? | x * 39500, SDS-PAGE | Pseudomonas putida |
1.13.11.19 | dimer | 2 * 50000 SDS-PAGE | Equus caballus |