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Literature summary extracted from

  • Yates, M.G.; Nason, A.
    Electron transport systems of the chemoautotroph Ferrobacillus ferrooxidans II. Purification and properties of a heat-labile iron-cytochrome c reductase (1966), J. Biol. Chem., 241, 4872-4880.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.9.98.1 2-oxo-3-phenylpropanoate 100% inhibition at 0.08 mM Acidithiobacillus ferrooxidans
1.9.98.1 Cr2+ slight inhibition Acidithiobacillus ferrooxidans
1.9.98.1 Cu2+ 100% inhibition at 0.1 mM Acidithiobacillus ferrooxidans
1.9.98.1 cysteine 100% inhibition at 0.2 mM Acidithiobacillus ferrooxidans
1.9.98.1 Fe3+ 48% inhibition at 1 mM Acidithiobacillus ferrooxidans
1.9.98.1 additional information not inhibitory: atebrin, amytal, antimycin A Acidithiobacillus ferrooxidans
1.9.98.1 Ni2+ slight inhibition Acidithiobacillus ferrooxidans
1.9.98.1 p-hydroxymercuribenzoate 100% inhibition at 0.5 mM, inhibition can be reversed by reduced glutathione or cysteine Acidithiobacillus ferrooxidans
1.9.98.1 reduced glutathione 67% inhibition at 1 mM Acidithiobacillus ferrooxidans
1.9.98.1 Sodium sulfide
-
 Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9.98.1 0.0275
-
 ferrocytochrome c
-
 Acidithiobacillus ferrooxidans
1.9.98.1 1.6
-
 Fe2+
-
 Acidithiobacillus ferrooxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.9.98.1 Fe hemoprotein Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.9.98.1 ferrocytochrome c + Fe3+ Acidithiobacillus ferrooxidans close association of enzyme with respiration chain ferricytochrome c + Fe2+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.9.98.1 Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.9.98.1 partial Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.9.98.1 0.006
-
-
 Acidithiobacillus ferrooxidans

Storage Stability

EC Number Storage Stability Organism
1.9.98.1 0-5°C, several weeks without loss of activity Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.9.98.1 ferrocytochrome c + Fe3+
-
 Acidithiobacillus ferrooxidans ferricytochrome c + Fe2+
-
 ?
1.9.98.1 ferrocytochrome c + Fe3+ close association of enzyme with respiration chain Acidithiobacillus ferrooxidans ferricytochrome c + Fe2+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.9.98.1 0.5
-
 several weeks, no loss of activity Acidithiobacillus ferrooxidans
1.9.98.1 22
-
 8 h, no loss of activity Acidithiobacillus ferrooxidans
1.9.98.1 40
-
 20 min, 10% loss of activity Acidithiobacillus ferrooxidans
1.9.98.1 60
-
 5 min, 46% loss of activity Acidithiobacillus ferrooxidans
1.9.98.1 90
-
 1 min, 74% loss of activity Acidithiobacillus ferrooxidans
1.9.98.1 100
-
 1 min, complete loss of activity Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.9.98.1 5.7 6.2
-
 Acidithiobacillus ferrooxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.9.98.1 5 6.7 pH 5: about 50% of maximum activity, pH 6.7: about 25% of maximum activity Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.9.98.1 cytochrome most highly purified fraction of the enzyme contains cytochrome c and apparently nonfunctional cytochrome b Acidithiobacillus ferrooxidans