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Literature summary extracted from

  • Levy, M.A.; Brandt, M.; Sheedy, K.M.; Holt, D.A.; Heaslip, J.I.; Trill, J.J.; Ryan, P.J.; Morris, R.A.; Garrison, L.M.; Bergsma, D.J.
    Cloning, expression and functional characterization of type 1 and type 2 steroid 5alpha-reductase from cynomolgus monkey: comparison with human and rat isoenzymes (1995), J. Steroid Biochem. Mol. Biol., 52, 307-319.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.22 monkey SR type 1 cDNA expression in CHO-cells Macaca fascicularis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.22 4-methyl-4-azasteroids competitive Homo sapiens
1.3.1.22 4-methyl-4-azasteroids competitive Macaca fascicularis
1.3.1.22 4-methyl-4-azasteroids competitive Rattus norvegicus
1.3.1.22 4-methyl-azasteroid
-
Homo sapiens
1.3.1.22 4-methyl-azasteroid
-
Macaca fascicularis
1.3.1.22 4-methyl-azasteroid
-
Rattus norvegicus
1.3.1.22 acrylate episteride
-
Homo sapiens
1.3.1.22 acrylate episteride
-
Macaca fascicularis
1.3.1.22 acrylate episteride
-
Rattus norvegicus
1.3.1.22 finasteride
-
Homo sapiens
1.3.1.22 finasteride
-
Macaca fascicularis
1.3.1.22 finasteride
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.22 0.0002
-
testosterone isoenzyme 2 Macaca fascicularis
1.3.1.22 0.0002
-
progesterone isoenzyme 2 Macaca fascicularis
1.3.1.22 0.0002
-
20alpha-hydroxyprogesterone isoenzyme 1 Homo sapiens
1.3.1.22 0.0003
-
progesterone isoenzyme 2 Homo sapiens
1.3.1.22 0.0003
-
progesterone isoenzyme 1 Homo sapiens
1.3.1.22 0.0003
-
progesterone isoenzyme 1 Macaca fascicularis
1.3.1.22 0.0004
-
androstenedione isoenzyme 2 Macaca fascicularis
1.3.1.22 0.0004
-
20alpha-hydroxyprogesterone isoenzyme 2 Homo sapiens
1.3.1.22 0.0005
-
20alpha-hydroxyprogesterone isoenzyme 1 Macaca fascicularis
1.3.1.22 0.0006
-
androstenedione isoenzyme 1 Macaca fascicularis
1.3.1.22 0.0007
-
testosterone isoenzyme 2 Homo sapiens
1.3.1.22 0.0008
-
androstenedione isoenzyme 2 Homo sapiens
1.3.1.22 0.001
-
androstenedione isoenzyme 1 Homo sapiens
1.3.1.22 0.002
-
corticosterone isoenzyme 2 Macaca fascicularis
1.3.1.22 0.002
-
testosterone isoenzyme 1 Macaca fascicularis
1.3.1.22 0.0035
-
testosterone isoenzyme 1 Homo sapiens
1.3.1.22 0.004
-
corticosterone isoenzyme 2 Homo sapiens
1.3.1.22 0.008
-
corticosterone isoenzyme 1 Macaca fascicularis
1.3.1.22 0.018
-
corticosterone isoenzyme 1 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.22 Homo sapiens
-
-
-
1.3.1.22 Macaca fascicularis
-
-
-
1.3.1.22 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.22 prostate gland
-
Homo sapiens
-
1.3.1.22 prostate gland
-
Rattus norvegicus
-
1.3.1.22 prostate gland
-
Macaca fascicularis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.22 20-alpha-hydroxypregn-4-ene-3one + NADPH
-
Homo sapiens 20-alpha-hydroxy-5alpha-pregnan-3-one + NADP+
-
?
1.3.1.22 20-alpha-hydroxypregn-4-ene-3one + NADPH
-
Macaca fascicularis 20-alpha-hydroxy-5alpha-pregnan-3-one + NADP+
-
?
1.3.1.22 androstenedione + NADPH + H+
-
Homo sapiens 5alpha-androstan-3,17-dione + NADP+
-
?
1.3.1.22 androstenedione + NADPH + H+
-
Rattus norvegicus 5alpha-androstan-3,17-dione + NADP+
-
?
1.3.1.22 androstenedione + NADPH + H+
-
Macaca fascicularis 5alpha-androstan-3,17-dione + NADP+
-
?
1.3.1.22 corticosterone + NADPH + H+
-
Homo sapiens 5alpha-dihydrocorticosterone + NADP+
-
r
1.3.1.22 corticosterone + NADPH + H+
-
Macaca fascicularis 5alpha-dihydrocorticosterone + NADP+
-
r
1.3.1.22 deoxycorticosterone + NADPH
-
Homo sapiens 21-hydroxy-5alpha-pregnan-3,20-dione
-
?
1.3.1.22 deoxycorticosterone + NADPH
-
Rattus norvegicus 21-hydroxy-5alpha-pregnan-3,20-dione
-
?
1.3.1.22 deoxycorticosterone + NADPH
-
Macaca fascicularis 21-hydroxy-5alpha-pregnan-3,20-dione
-
?
1.3.1.22 progesterone + NADPH + H+
-
Homo sapiens 5alpha-pregnan-3,20-dione + NADP+
-
?
1.3.1.22 progesterone + NADPH + H+
-
Rattus norvegicus 5alpha-pregnan-3,20-dione + NADP+
-
?
1.3.1.22 progesterone + NADPH + H+
-
Macaca fascicularis 5alpha-pregnan-3,20-dione + NADP+
-
?
1.3.1.22 testosterone + NADPH + H+
-
Homo sapiens 17beta-hydroxy-5alpha-androstan-3-one + NADP+
-
?
1.3.1.22 testosterone + NADPH + H+
-
Rattus norvegicus 17beta-hydroxy-5alpha-androstan-3-one + NADP+
-
?
1.3.1.22 testosterone + NADPH + H+
-
Macaca fascicularis 17beta-hydroxy-5alpha-androstan-3-one + NADP+
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.22 5
-
recombinant monkey 5alpha-reductase isozyme 2 Macaca fascicularis
1.3.1.22 5
-
recombinant human 5alpha-reductase isozyme 2 Homo sapiens
1.3.1.22 7.5
-
recombinant human 5alpha-reductase isozyme 1 Homo sapiens
1.3.1.22 8
-
recombinant monkey 5alpha-reductase isozyme 1 Macaca fascicularis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.22 4.5 9.5 recombinant human 5alpha-reductase isozyme 2 Homo sapiens
1.3.1.22 4.5 7.5 recombinant monkey 5alpha-reductase isozyme 2 Macaca fascicularis
1.3.1.22 4.5 9 recombinant human 5alpha-reductase isozyme 1 Homo sapiens
1.3.1.22 5.5 9 recombinant monkey 5alpha-reductase isozyme 1 Macaca fascicularis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.22 0.0000003
-
acylate episteride iosenzyme 2 Homo sapiens
1.3.1.22 0.0000005
-
acylate episteride iosenzyme 2 Macaca fascicularis
1.3.1.22 0.000001
-
finasteride iosenzyme 2 Homo sapiens
1.3.1.22 0.000002
-
4-methyl-azasteroid iosenzyme 2 Macaca fascicularis
1.3.1.22 0.000003
-
4-methyl-azasteroid iosenzyme 1 Homo sapiens
1.3.1.22 0.000003
-
4-methyl-azasteroid iosenzyme 1 Macaca fascicularis
1.3.1.22 0.000003
-
4-methyl-azasteroid iosenzyme 2 Rattus norvegicus
1.3.1.22 0.000004
-
finasteride iosenzyme 2 Rattus norvegicus
1.3.1.22 0.000004
-
4-methyl-azasteroid iosenzyme 2 Homo sapiens
1.3.1.22 0.000004
-
acylate episteride iosenzyme 2 Rattus norvegicus
1.3.1.22 0.000005
-
finasteride iosenzyme 2 Macaca fascicularis
1.3.1.22 0.000006
-
finasteride iosenzyme 1 Rattus norvegicus
1.3.1.22 0.000008
-
4-methyl-azasteroid iosenzyme 1 Rattus norvegicus
1.3.1.22 0.00002
-
acylate episteride iosenzyme 1 Rattus norvegicus
1.3.1.22 0.00022
-
finasteride iosenzyme 1 Macaca fascicularis
1.3.1.22 0.0005
-
acylate episteride iosenzyme 1 Macaca fascicularis
1.3.1.22 0.001
-
finasteride iosenzyme 1 Homo sapiens
1.3.1.22 0.0041
-
acylate episteride iosenzyme 1 Homo sapiens