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Literature summary extracted from

  • Tsukita, S.; Koyanagi, S.; Nagata, K.; Koizuka, H.; Akashi, H.; Shimoyama, T.; Tamura, T.; Sone, N.
    Characterization of a cb-type cytochrome c oxidase from Helicobacter pylori (1999), J. Biochem., 125, 194-201.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.9 CN-
-
Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.9 0.00004
-
O2
-
Helicobacter pylori
7.1.1.9 0.0009
-
ferrocytochrome c553
-
Helicobacter pylori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.9 membrane
-
Helicobacter pylori 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.9 26000
-
1 * 58000 + 1 * 26000, 58000 Da band may be composed of 2 subunits of the cb-type oxidase, the 26000 Da subunit may be a heme c bearing diheme or mono-heme of the enzyme, SDS-PAGE Helicobacter pylori
7.1.1.9 58000
-
1 * 58000 + 1 * 26000, 58000 Da band may be composed of 2 subunits of the cb-type oxidase, the 26000 Da subunit may be a heme c bearing diheme or mono-heme of the enzyme, SDS-PAGE Helicobacter pylori
7.1.1.9 200000
-
gel filtration Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.9 Triton X-100, Q-Sepharose, chelating Sepharose, gel filtration Helicobacter pylori

Storage Stability

EC Number Storage Stability Organism
7.1.1.9 -80°C, no severe loss of activity Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 Saccharomyces cerevisiae cytochrome c Helicobacter pylori ferricytochrome c + H2O
-
?
7.1.1.9 ferrocytochrome c + O2 horse ferrocytochrome c Helicobacter pylori ferricytochrome c + H2O
-
?
7.1.1.9 ferrocytochrome c + O2 artificial electron donor: ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine Helicobacter pylori ferricytochrome c + H2O
-
?
7.1.1.9 ferrocytochrome c553 + O2 + H+
-
Helicobacter pylori ferricytochrome c553 + H2O
-
r

Subunits

EC Number Subunits Comment Organism
7.1.1.9 dimer 1 * 58000 + 1 * 26000, 58000 Da band may be composed of 2 subunits of the cb-type oxidase, the 26000 Da subunit may be a heme c bearing diheme or mono-heme of the enzyme, SDS-PAGE Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.9 252
-
ferrocytochrome c553
-
Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.9 heme c enzyme contains 3 heme c Helicobacter pylori
7.1.1.9 protoheme
-
Helicobacter pylori

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.1.1.9 0.0026
-
CN-
-
Helicobacter pylori