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Literature summary extracted from

  • Anemuller, S.; Schäfer, G.
    Cytochrome aa3 from Sulfolobus acidocaldarius. A single-subunit, quinol-oxidizing archaebacterial terminal oxidase (1990), Eur. J. Biochem., 191, 297-305.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.4 azide 20 mM, complete inhibition Sulfolobus acidocaldarius
7.1.1.4 Cl- 70 mM, 50% inhibition Sulfolobus acidocaldarius
7.1.1.4 cyanide 1-3 mM, complete inhibition Sulfolobus acidocaldarius
7.1.1.4 phosphate 23 mM, 50% inhibition Sulfolobus acidocaldarius
7.1.1.4 SO42- 28 mM, 50% inhibition Sulfolobus acidocaldarius
7.1.1.4 Sulfide 1-3 mM, complete inhibition Sulfolobus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.4 0.1
-
 N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride pH 7.0, 40°C Sulfolobus acidocaldarius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.4 Cu2+ the single subunit (38000-40000 Da) contains two copper ions Sulfolobus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.4 38000
-
 x * 38000 Sulfolobus acidocaldarius
7.1.1.4 100000 104000 gel filtration Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.4 caldariellaquinol + O2 + n H+/in Sulfolobus acidocaldarius
-
 caldariellaquinone + H2O + n H+/out
-
 ?
7.1.1.4 caldariellaquinol + O2 + n H+/in Sulfolobus acidocaldarius DSM 639
-
 caldariellaquinone + H2O + n H+/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.4 Sulfolobus acidocaldarius
-
-
-
7.1.1.4 Sulfolobus acidocaldarius DSM 639
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.4
-
 Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.4 36
-
 pH 7.0, 40°C, oxidation of N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.4 caldariella quinol + O2 + n H+/in isolated aa3 preparation does not oxidize cytochrome c Sulfolobus acidocaldarius caldariella quinone + H2O + n H+/out
-
 ?
7.1.1.4 caldariella quinol + O2 + n H+/in isolated aa3 preparation does not oxidize cytochrome c Sulfolobus acidocaldarius DSM 639 caldariella quinone + H2O + n H+/out
-
 ?
7.1.1.4 caldariellaquinol + O2 + n H+/in
-
 Sulfolobus acidocaldarius caldariellaquinone + H2O + n H+/out
-
 ?
7.1.1.4 caldariellaquinol + O2 + n H+/in
-
 Sulfolobus acidocaldarius DSM 639 caldariellaquinone + H2O + n H+/out
-
 ?
7.1.1.4 N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride + O2
-
 Sulfolobus acidocaldarius ?
-
 ?
7.1.1.4 N,N,N',N'-tetramethyl-1,4-phenylenediamine hydrochloride + O2
-
 Sulfolobus acidocaldarius DSM 639 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.4 ? x * 38000 Sulfolobus acidocaldarius

Synonyms

EC Number Synonyms Comment Organism
7.1.1.4 cytochrome aa3
-
 Sulfolobus acidocaldarius

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.4 heme hemeprotein. The single subunit (38000-40000 Da) contains two heme a molecules. The redox potentials of the heme centres are +220 mV and +370 mV, respectively. One heme is a heme-a3 centre Sulfolobus acidocaldarius