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Literature summary extracted from
- Purification and characterization of a novel cyclohexylamine oxidase from the cyclohexylamine-degrading Brevibacterium oxydans IH-35A (1999), J. Biosci. Bioeng., 88, 264-268.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.3.12 | Quinacrine | - |
Microbacterium oxydans |  |
| 1.4.3.12 | Quinine | - |
Microbacterium oxydans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.12 | 1.23 | - |
Cyclohexylamine | - |
Microbacterium oxydans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.12 | 50000 | - |
gel filtration | Microbacterium oxydans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.3.12 | cyclohexylamine + O2 + H2O | Microbacterium oxydans | highly specific for alicyclic monoamines and aliphatic monoamines | cyclohexanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | cyclohexylamine + O2 + H2O | Microbacterium oxydans IH-35A | highly specific for alicyclic monoamines and aliphatic monoamines | cyclohexanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | cyclopentylamine + O2 + H2O | Microbacterium oxydans | highly specific for alicyclic monoamines and aliphatic monoamines | cyclopentanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | cyclopentylamine + O2 + H2O | Microbacterium oxydans IH-35A | highly specific for alicyclic monoamines and aliphatic monoamines | cyclopentanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | N-methylcyclohexylamine + O2 + H2O | Microbacterium oxydans | highly specific for alicyclic monoamines and aliphatic monoamines | cyclohexanone + methylamine + H2O2 | - |
? | |
| 1.4.3.12 | N-methylcyclohexylamine + O2 + H2O | Microbacterium oxydans IH-35A | highly specific for alicyclic monoamines and aliphatic monoamines | cyclohexanone + methylamine + H2O2 | - |
? | |
| 1.4.3.12 | secondary butylamine + O2 + H2O | Microbacterium oxydans | highly specific for alicyclic monoamines and aliphatic monoamines | butanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | secondary butylamine + O2 + H2O | Microbacterium oxydans IH-35A | highly specific for alicyclic monoamines and aliphatic monoamines | butanone + NH3 + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.3.12 | Microbacterium oxydans | - |
- |
- |
| 1.4.3.12 | Microbacterium oxydans IH-35A | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.4.3.12 | flavoprotein | - |
Microbacterium oxydans |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.3.12 | to homogeneity, chromatography steps | Microbacterium oxydans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.4.3.12 | cell culture | inducible by growth on cyclohexylamine | Microbacterium oxydans | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.3.12 | cyclohexylamine + O2 + H2O | highly specific for alicyclic monoamines and aliphatic monoamines | Microbacterium oxydans | cyclohexanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | cyclohexylamine + O2 + H2O | highly specific for alicyclic monoamines and aliphatic monoamines | Microbacterium oxydans IH-35A | cyclohexanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | cyclopentylamine + O2 + H2O | highly specific for alicyclic monoamines and aliphatic monoamines | Microbacterium oxydans | cyclopentanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | cyclopentylamine + O2 + H2O | highly specific for alicyclic monoamines and aliphatic monoamines | Microbacterium oxydans IH-35A | cyclopentanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | N-methylcyclohexylamine + O2 + H2O | highly specific for alicyclic monoamines and aliphatic monoamines | Microbacterium oxydans | cyclohexanone + methylamine + H2O2 | - |
? | |
| 1.4.3.12 | N-methylcyclohexylamine + O2 + H2O | highly specific for alicyclic monoamines and aliphatic monoamines | Microbacterium oxydans IH-35A | cyclohexanone + methylamine + H2O2 | - |
? | |
| 1.4.3.12 | secondary butylamine + O2 + H2O | highly specific for alicyclic monoamines and aliphatic monoamines | Microbacterium oxydans | butanone + NH3 + H2O2 | - |
? | |
| 1.4.3.12 | secondary butylamine + O2 + H2O | highly specific for alicyclic monoamines and aliphatic monoamines | Microbacterium oxydans IH-35A | butanone + NH3 + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.4.3.12 | monomer | 1 * 50000, SDS-PAGE | Microbacterium oxydans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.12 | 30 | - |
stable up to | Microbacterium oxydans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.12 | 7.4 | - |
- |
Microbacterium oxydans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.12 | 6 | 7 | stable between | Microbacterium oxydans |
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