EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.7.1.3 | additional information | nit-3 mutant (FGSC 262): reduced FAD-nitrate reductase and reduced methylviologen-nitrate reductase activities | Neurospora crassa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.3 | p-hydroxymercuribenzoate | at 0.1 mM complete inhibition | Neurospora crassa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.1.3 | 235000 | - |
sucrose density gradient centrifugation, gel filtration | Neurospora crassa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.3 | nitrate + NADPH | Neurospora crassa | - |
nitrite + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.1.3 | Neurospora crassa | - |
wild-type STA4, FGSC 358 and nit-1 mutant, FGSC 262 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.1.3 | partially purified | Neurospora crassa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.7.1.3 | nitrite + NADP+ + H2O = nitrate + NADPH + H+ | sulfhydryl groups may participate in the binding of the protein subunits | Neurospora crassa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.1.3 | 8.55 | - |
- |
Neurospora crassa |
EC Number | Storage Stability | Organism |
---|---|---|
1.7.1.3 | -20°C, stable for 6 months or longer, 5% loss of activity | Neurospora crassa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.3 | nitrate + NADPH | - |
Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
1.7.1.3 | nitrate + NADPH | associated FAD-nitrate reductase and methylviologen-nitrate activity | Neurospora crassa | nitrite + NADP+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.1.3 | More | at least 2 subunits suggested | Neurospora crassa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.1.3 | 60 | - |
labile, loss of acitvity after 2 min | Neurospora crassa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.1.3 | additional information | - |
optima of associated activities | Neurospora crassa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.3 | cytochrome b557 | - |
Neurospora crassa | |
1.7.1.3 | FAD | - |
Neurospora crassa |