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Literature summary extracted from

  • Snoep, J.L.; Teixeira de Mattos, M.J.; Starrenburg, M.J.C.; Hugenholtz, J.
    Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis (1992), J. Bacteriol., 174, 4838.-4841.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 EDTA
-
 Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 50
-
 pyruvate
-
 Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 62000
-
 x * 62000, SDS-PAGE Lactococcus lactis
2.2.1.6 172000
-
 gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 pyruvate Lactococcus lactis constitutive high expression, the enzyme is active only under conditions of pyruvate excess ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Lactococcus lactis
-
 subsp. lactis bv, diacetylactis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 additional information
-
-
 Lactococcus lactis

Storage Stability

EC Number Storage Stability Organism
2.2.1.6 -20°C, 20% glycerol, stable for 2 months Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Lactococcus lactis 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate constitutive high expression, the enzyme is active only under conditions of pyruvate excess Lactococcus lactis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 ? x * 62000, SDS-PAGE Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 6
-
-
 Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 thiamine diphosphate required as cofactor Lactococcus lactis
2.2.1.6 thiamine diphosphate Km: 0.0032 mM Lactococcus lactis