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Literature summary extracted from

  • Chang, A.K.; Duggleby, R.G.
    Expression, purification and characterization of Arabidopsis thaliana acetohydroxyacid synthase (1997), Biochem. J., 327, 161-169.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 cloned into the bacterial expression vector pT7-7 and expressed in Escherichia coli BL21(DE3) Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 chlorsulphuron
-
 Arabidopsis thaliana
2.2.1.6 Ile insensitive to Arabidopsis thaliana
2.2.1.6 imazapyr
-
 Arabidopsis thaliana
2.2.1.6 Leu insensitive to Arabidopsis thaliana
2.2.1.6 Val no inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 additional information
-
 additional information non-hyperbolic substrate-saturation curve, involving interaction between the active sites of the dimer Arabidopsis thaliana
2.2.1.6 8.01
-
 pyruvate Km-value of the first active site Arabidopsis thaliana
2.2.1.6 100
-
 pyruvate Km-value of the second active site Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ required Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 61000
-
 2 * 61000, SDS-PAGE Arabidopsis thaliana
2.2.1.6 63864
-
 2 * 63864, ion spray MS analysis Arabidopsis thaliana
2.2.1.6 109000
-
 gel filtration in absence of FAD Arabidopsis thaliana
2.2.1.6 113000
-
 gel filtration in presence of FAD Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 pyruvate Arabidopsis thaliana first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 7.88
-
-
 Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
2.2.1.6 -70°C, 4% loss of activity after 3 weeks with three freeze-thaw cycles, 8% loss of activity after 5 weeks with five freeze-thaw cycles, 9-10% loss of activity after 6 months with no intervening freeze-thaw cycle Arabidopsis thaliana
2.2.1.6 4°C, 10% loss of activity after 3 weeks, 23% loss of activity after 5 weeks Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Arabidopsis thaliana 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 dimer 2 * 61000, SDS-PAGE Arabidopsis thaliana
2.2.1.6 dimer 2 * 63864, ion spray MS analysis Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 6.5 8.5
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD required as cofactor Arabidopsis thaliana
2.2.1.6 thiamine diphosphate required as cofactor Arabidopsis thaliana