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Literature summary extracted from

  • Arfin, S.M.; Koziell, D.A.
    Acetolactate synthase of Pseudomonas aeruginosa I. Purification and allosteric properties (1973), Biochim. Biophys. Acta, 321, 348-355.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 Ile
-
 Pseudomonas aeruginosa
2.2.1.6 Leu
-
 Pseudomonas aeruginosa
2.2.1.6 Val
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ required Pseudomonas aeruginosa
2.2.1.6 Mg2+ stimulates Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 600000
-
 equilibrium sedimentation Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 25.6
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
2.2.1.6 0-4°C, 50 mM potassium phosphate, pH 7.0, 10 mM mercaptoethanol, 1 mM MgCl2, 0.01 mM thiamine diphosphate, 1 month, 20% loss of activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Pseudomonas aeruginosa 2-acetolactate + CO2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 8 8.8
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD no stimulation Pseudomonas aeruginosa
2.2.1.6 thiamine diphosphate stimulates Pseudomonas aeruginosa