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Literature summary extracted from

  • DeFelice, M.; Squires, C.; Levinthal, M.
    A comparative study of the acetohydroxy acid synthase isoenzymes of Escherichia coli K-12 (1978), Biochim. Biophys. Acta, 541, 9-17.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.2.1.6 dialysis causes 38% loss of activity of isoenzyme I, stimulates activity of isoenzyme III Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 2-oxobutanoate isoenzyme I has lower sensitivity to inhibition than isoenzyme III Escherichia coli
2.2.1.6 Val isoenzyme I is more resistant to inhibition than isoenzyme III Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 1.3
-
pyruvate isoenzyme I Escherichia coli
2.2.1.6 7.6
-
pyruvate isoenzyme III Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ required Escherichia coli
2.2.1.6 Mg2+ Km: 0.3 mM for isoenzyme I, 0.4 mM for isoenzyme III Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Escherichia coli
-
K-12
-
2.2.1.6 Escherichia coli
-
wild type and two isogenic strains PS1035, containing only acetohydroxy acid synthase III, and strain PS1036, containing only acetohydroxy acid synthase I
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
Escherichia coli 2-acetolactate + CO2
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.6 45
-
15 min, 69% loss of activity, isoenzyme I, slight stimulation of activity of isoenzyme III Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD required by isoenzyme I, no requirement by isoenzyme III Escherichia coli
2.2.1.6 FAD Km: 0.00016 mM for isoenzyme I Escherichia coli
2.2.1.6 thiamine diphosphate required as cofactor Escherichia coli
2.2.1.6 thiamine diphosphate Km: 0.0087 mM for isoenzyme I, 0.026 mM for isoenzyme III Escherichia coli