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Literature summary extracted from

  • Singh, B.K.; Stidham, M.A.; Shaner, D.L.
    Separation and characterization of two forms of acetohydroxy acid synthase from black mexican sweet corn cells (1988), J. Chromatogr., 444, 251-261.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 Ile enzyme form AHS I is inhibited. Enzyme form AHS II is not inhibited Zea mays
2.2.1.6 imazapyr enzyme form AHS I and AHS II Zea mays
2.2.1.6 Leu enzyme form AHS I is inhibited. Enzyme form AHS II is not inhibited Zea mays
2.2.1.6 sulfometuron-methyl enzyme form AHS I and AHS II Zea mays
2.2.1.6 Val enzyme form AHS I is inhibited. Enzyme form AHS II is not inhibited Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 5
-
 pyruvate AHAS I Zea mays
2.2.1.6 8
-
 pyruvate AHAS II Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 55000
-
 AHAS II, gel filtration Zea mays
2.2.1.6 193000
-
 AHAS I, gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 pyruvate Zea mays first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Zea mays
-
 black mexican sweet, two enzyme forms: AHAS I and AHAS II
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.2.1.6 cell suspension culture
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Zea mays 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile Zea mays ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 6 7 AHAS I Zea mays
2.2.1.6 7
-
 AHAS II Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.6 5.5 8 pH 5.5: about 80% of maximal activity, pH 8.0: about 55% of maximal activity, AHAS I Zea mays
2.2.1.6 6.5 7.5 about 60% of maximal activity at pH 6.5 and pH 7.5, AHAS II Zea mays