BRENDA - Enzyme Database

Properties of crystalline L-aspartate beta-decarboxylase of Pseudomonas dacunhae

Shibatani, T.; Kakimoto, T.; Kato, J.; Nishimura, N.; Chibata, I.; J. Ferment. Technol. 52, 886-898 (1974)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.12
2-oxoglutarate
activates
Pseudomonas dacunhae
4.1.1.12
DL-erythro-3-hydroxyaspartate
-
Pseudomonas dacunhae
4.1.1.12
DL-threo-3-hydroxyaspartate
-
Pseudomonas dacunhae
4.1.1.12
oxalacetate
activates
Pseudomonas dacunhae
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.1.1.12
-
Pseudomonas dacunhae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.12
fumarate
-
Pseudomonas dacunhae
4.1.1.12
L-cysteine sulfinate
-
Pseudomonas dacunhae
4.1.1.12
malate
-
Pseudomonas dacunhae
4.1.1.12
Maleate
-
Pseudomonas dacunhae
4.1.1.12
malonate
-
Pseudomonas dacunhae
4.1.1.12
succinate
-
Pseudomonas dacunhae
4.1.1.12
Tartrate
-
Pseudomonas dacunhae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.12
1
-
L-Asp
in presence of 2-oxoglutarate or pyridoxal 5'-phosphate
Pseudomonas dacunhae
4.1.1.12
5
-
L-Asp
in absence of cofactors
Pseudomonas dacunhae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.12
Pseudomonas dacunhae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.12
L-Asp
highly specific for L-Asp
3958
Pseudomonas dacunhae
L-Ala + CO2
-
3958
Pseudomonas dacunhae
-
4.1.1.12
L-Cysteinesulfinate
-
3958
Pseudomonas dacunhae
L-Ala + SO2
-
-
-
-
4.1.1.12
additional information
transamination between Asp and 2-oxoglutarate
3958
Pseudomonas dacunhae
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.12
30
-
cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4.1.1.12
47
-
decarboxylation and transamination
Pseudomonas dacunhae
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.1.12
20
40
20°C: about 60% of maximal activity, 40°C: about 20% of maximal activity, cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4.1.1.12
30
50
30°C: about 50% of maximal activity, 50°C: about 65% of maximal activity, aspartate decarboxylase activity
Pseudomonas dacunhae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.12
2.5
-
transamination between Asp and 2-oxoglutarate
Pseudomonas dacunhae
4.1.1.12
4
-
cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4.1.1.12
5.3
-
aspartate decarboxylase activity
Pseudomonas dacunhae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.12
3
5
pH 3: about 35% of maximal activity, pH 5: about 40% of maximal activity, cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4.1.1.12
4
6
about 20% of maximal activity at pH 4 and pH 6, aspartate decarboxylase activity
Pseudomonas dacunhae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.12
pyridoxal 5'-phosphate
activates
Pseudomonas dacunhae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.12
2-oxoglutarate
activates
Pseudomonas dacunhae
4.1.1.12
DL-erythro-3-hydroxyaspartate
-
Pseudomonas dacunhae
4.1.1.12
DL-threo-3-hydroxyaspartate
-
Pseudomonas dacunhae
4.1.1.12
oxalacetate
activates
Pseudomonas dacunhae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.12
pyridoxal 5'-phosphate
activates
Pseudomonas dacunhae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.1.1.12
-
Pseudomonas dacunhae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.12
fumarate
-
Pseudomonas dacunhae
4.1.1.12
L-cysteine sulfinate
-
Pseudomonas dacunhae
4.1.1.12
malate
-
Pseudomonas dacunhae
4.1.1.12
Maleate
-
Pseudomonas dacunhae
4.1.1.12
malonate
-
Pseudomonas dacunhae
4.1.1.12
succinate
-
Pseudomonas dacunhae
4.1.1.12
Tartrate
-
Pseudomonas dacunhae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.12
1
-
L-Asp
in presence of 2-oxoglutarate or pyridoxal 5'-phosphate
Pseudomonas dacunhae
4.1.1.12
5
-
L-Asp
in absence of cofactors
Pseudomonas dacunhae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.12
L-Asp
highly specific for L-Asp
3958
Pseudomonas dacunhae
L-Ala + CO2
-
3958
Pseudomonas dacunhae
-
4.1.1.12
L-Cysteinesulfinate
-
3958
Pseudomonas dacunhae
L-Ala + SO2
-
-
-
-
4.1.1.12
additional information
transamination between Asp and 2-oxoglutarate
3958
Pseudomonas dacunhae
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.12
30
-
cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4.1.1.12
47
-
decarboxylation and transamination
Pseudomonas dacunhae
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.1.12
20
40
20°C: about 60% of maximal activity, 40°C: about 20% of maximal activity, cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4.1.1.12
30
50
30°C: about 50% of maximal activity, 50°C: about 65% of maximal activity, aspartate decarboxylase activity
Pseudomonas dacunhae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.12
2.5
-
transamination between Asp and 2-oxoglutarate
Pseudomonas dacunhae
4.1.1.12
4
-
cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4.1.1.12
5.3
-
aspartate decarboxylase activity
Pseudomonas dacunhae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.12
3
5
pH 3: about 35% of maximal activity, pH 5: about 40% of maximal activity, cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4.1.1.12
4
6
about 20% of maximal activity at pH 4 and pH 6, aspartate decarboxylase activity
Pseudomonas dacunhae