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Literature summary extracted from

  • Shibatani, T.; Kakimoto, T.; Kato, J.; Nishimura, N.; Chibata, I.
    Properties of crystalline L-aspartate beta-decarboxylase of Pseudomonas dacunhae (1974), J. Ferment. Technol., 52, 886-898.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.12 2-oxoglutarate activates Pseudomonas dacunhae
4.1.1.12 DL-erythro-3-hydroxyaspartate
-
 Pseudomonas dacunhae
4.1.1.12 DL-threo-3-hydroxyaspartate
-
 Pseudomonas dacunhae
4.1.1.12 oxalacetate activates Pseudomonas dacunhae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.12
-
 Pseudomonas dacunhae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.12 fumarate
-
 Pseudomonas dacunhae
4.1.1.12 L-cysteine sulfinate
-
 Pseudomonas dacunhae
4.1.1.12 malate
-
 Pseudomonas dacunhae
4.1.1.12 Maleate
-
 Pseudomonas dacunhae
4.1.1.12 malonate
-
 Pseudomonas dacunhae
4.1.1.12 succinate
-
 Pseudomonas dacunhae
4.1.1.12 Tartrate
-
 Pseudomonas dacunhae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.12 1
-
 L-Asp in presence of 2-oxoglutarate or pyridoxal 5'-phosphate Pseudomonas dacunhae
4.1.1.12 5
-
 L-Asp in absence of cofactors Pseudomonas dacunhae

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.12 Pseudomonas dacunhae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.12 L-Asp highly specific for L-Asp Pseudomonas dacunhae L-Ala + CO2
-
 ?
4.1.1.12 L-Cysteinesulfinate
-
 Pseudomonas dacunhae L-Ala + SO2
-
 ?
4.1.1.12 additional information transamination between Asp and 2-oxoglutarate Pseudomonas dacunhae ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.12 30
-
 cysteine sulfinate desulfinase activity Pseudomonas dacunhae
4.1.1.12 47
-
 decarboxylation and transamination Pseudomonas dacunhae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.12 20 40 20°C: about 60% of maximal activity, 40°C: about 20% of maximal activity, cysteine sulfinate desulfinase activity Pseudomonas dacunhae
4.1.1.12 30 50 30°C: about 50% of maximal activity, 50°C: about 65% of maximal activity, aspartate decarboxylase activity Pseudomonas dacunhae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.12 2.5
-
 transamination between Asp and 2-oxoglutarate Pseudomonas dacunhae
4.1.1.12 4
-
 cysteine sulfinate desulfinase activity Pseudomonas dacunhae
4.1.1.12 5.3
-
 aspartate decarboxylase activity Pseudomonas dacunhae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.12 3 5 pH 3: about 35% of maximal activity, pH 5: about 40% of maximal activity, cysteine sulfinate desulfinase activity Pseudomonas dacunhae
4.1.1.12 4 6 about 20% of maximal activity at pH 4 and pH 6, aspartate decarboxylase activity Pseudomonas dacunhae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.12 pyridoxal 5'-phosphate activates Pseudomonas dacunhae