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Literature summary extracted from

  • Kunapuli, S.P.; Vaidyanathan, C.S.
    Purification and characterization of a new indole oxygenase from the leaves of Tecoma stans L (1983), Plant Physiol., 71, 19-23.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.13.11.17 dialysis, 8 h, results in complete loss of activity Tecoma stans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.17 azide 0.5 mM, 54% inhibition Tecoma stans
1.13.11.17 CO 0.5 mM, 58% inhibition Tecoma stans
1.13.11.17 Cu2+ 1 mM, CuSO4, 25% inhibition Tecoma stans
1.13.11.17 cyanide 0.5 mM, 64% inhibition Tecoma stans
1.13.11.17 Fe2+ 1 mM, FeSO4, 50% inhibition Tecoma stans
1.13.11.17 Fe3+
-
 Tecoma stans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.17 0.0189
-
 indole
-
 Tecoma stans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.17 soluble
-
 Tecoma stans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.17 58000
-
 gel filtration Tecoma stans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.17 indole + O2 Tecoma stans during daytime activity is less, it reaches a peak during nighttime ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.17 Tecoma stans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.17
-
 Tecoma stans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.17 leaf
-
 Tecoma stans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.17 0.0157
-
 disappearance of 1 nmol indole in 1 min Tecoma stans

Storage Stability

EC Number Storage Stability Organism
1.13.11.17 -20°C, 18 h without appreciable loss of activity, complete loss of activity after 48 h Tecoma stans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.17 5-bromoindole + O2 15% of the activity with indole Tecoma stans 5-bromo-2-formylaminobenzaldehyde
-
 ?
1.13.11.17 5-hydroxyindole + O2 20% of the activity with indole Tecoma stans 2-formylamino-5-hydroxybenzaldehyde
-
 ?
1.13.11.17 5-methylindole + O2 26% of the activity with indole Tecoma stans 2-formylamino-5-methylbenzaldehyde
-
 ?
1.13.11.17 indole + O2 conversion of indole to anthranilic acid Tecoma stans anthranilic acid
-
 ?
1.13.11.17 indole + O2 during daytime activity is less, it reaches a peak during nighttime Tecoma stans ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.17 30
-
-
 Tecoma stans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.17 5.2
-
-
 Tecoma stans