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Literature summary extracted from

  • Ishimura, Y.
    L-Tryptophan 2,3-dioxygenase (tryptophan pyrrolase) (Pseudomonas fluorescens) (1970), Methods Enzymol., 17A, 429-434.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.52 alpha-methyl-DL-tryptophan can activate the enzyme when lower concentrations of L-Trp are used Pseudomonas fluorescens
1.13.11.52 ascorbic acid the ferric enzyme is incapable of catalyzing the reaction but is reduced to an active ferrous form by the addition of ascorbic acid and L-Trp in presence of O2 Pseudomonas fluorescens
1.13.11.52 Cys can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
1.13.11.52 DTT can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
1.13.11.52 ethylhydroperoxide can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
1.13.11.52 GSH can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
1.13.11.52 H2O2 can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
1.13.11.52 methylhydroperoxide can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.52 ferricyanide
-
Pseudomonas fluorescens
1.13.11.52 hydroxylamine
-
Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 0.06
-
O2
-
Pseudomonas fluorescens
1.13.11.52 0.3
-
L-Trp
-
Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.52 copper contains copper in significant quantity Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52
-
Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.52 5.3
-
-
Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.13.11.52 0°C, under anaerobic conditions in presence of tryptophan, stable for several days Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 L-Trp + O2 absolutely specific for L-Trp Pseudomonas fluorescens L-formylkynurenine
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.52 7.2
-
-
Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.52 6.5
-
most stable at Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.52 heme contains protohematin IX as a prosthetic group Pseudomonas fluorescens