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Literature summary extracted from

  • Dang, Y.; Dale, W.E.; Brown, O.R.
    Comparative effects of oxygen on indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase of the kynurenine pathway (2000), Free Radic. Biol. Med., 28, 615-624.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.52 ascorbic acid tryptophan 2,3-dioxygenase: maximal activity at 10 mM ascorbate Rattus norvegicus
1.13.11.52 hyperbaric oxygen
-
 Rattus norvegicus
1.13.11.52 hyperbaric oxygen
-
 Meriones unguiculatus
1.13.11.52 O2-
-
 Rattus norvegicus
1.13.11.52 O2-
-
 Meriones unguiculatus

Application

EC Number Application Comment Organism
1.13.11.52 medicine rate-limiting enzyme in kynurenine pathway Rattus norvegicus
1.13.11.52 medicine rate-limiting enzyme in kynurenine pathway Meriones unguiculatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 2.8
-
 O2 indoleamine 2,3-dioxygenase Meriones unguiculatus
1.13.11.52 2.8
-
 oxygen pH 6.5, 37°C Rattus norvegicus
1.13.11.52 2.8
-
 oxygen pH 6.5, 37°C Meriones unguiculatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.52 D-tryptophan + O2 Rattus norvegicus
-
 N-formyl-D-kynurenine
-
 ?
1.13.11.52 D-tryptophan + O2 Meriones unguiculatus
-
 N-formyl-D-kynurenine
-
 ?
1.13.11.52 L-Trp + O2 Rattus norvegicus indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid L-formylkynurenine
-
 ?
1.13.11.52 L-Trp + O2 Meriones unguiculatus indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid L-formylkynurenine
-
 ?
1.13.11.52 L-tryptophan + O2 Rattus norvegicus no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2 Meriones unguiculatus no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2- Rattus norvegicus
-
 N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2- Meriones unguiculatus
-
 N-formyl-L-kynurenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Meriones unguiculatus
-
-
-
1.13.11.52 Meriones unguiculatus
-
 indoleamine 2,3-dioxygenase
-
1.13.11.52 Rattus norvegicus
-
 Sprague Dawley
-
1.13.11.52 Rattus norvegicus
-
 indoleamine 2,3-dioxygenase from small intestine
-
1.13.11.52 Rattus norvegicus
-
 tryptophan 2,3-dioxygenase from liver
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52 tissues thawed at room temperature, homogenized in 0.14 M potassium chloride plus 20 mM potassium phosphate, pH 7.0 Rattus norvegicus
1.13.11.52 tissues thawed at room temperature, homogenized in 0.14 M potassium chloride plus 20 mM potassium phosphate, pH 7.0 Meriones unguiculatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.52 brain
-
 Rattus norvegicus
-
1.13.11.52 brain
-
 Meriones unguiculatus
-
1.13.11.52 brain indoleamine 2,3-dioxygenase Meriones unguiculatus
-
1.13.11.52 liver tryptophan 2,3-dioxygenase Rattus norvegicus
-
1.13.11.52 lung
-
 Meriones unguiculatus
-
1.13.11.52 lung indoleamine 2,3-dioxygenase Meriones unguiculatus
-
1.13.11.52 small intestine
-
 Rattus norvegicus
-
1.13.11.52 small intestine indoleamine 2,3-dioxygenase Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.52 0.011
-
 brain, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C Meriones unguiculatus
1.13.11.52 0.014
-
 brain, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C Meriones unguiculatus
1.13.11.52 0.07
-
 brain, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C Meriones unguiculatus
1.13.11.52 0.145
-
 small intestine, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C Rattus norvegicus
1.13.11.52 0.198
-
 lung, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C Meriones unguiculatus
1.13.11.52 0.24
-
 small intestine, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C Rattus norvegicus
1.13.11.52 0.38
-
 small intestine, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C Rattus norvegicus
1.13.11.52 0.873
-
 lung, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C Meriones unguiculatus
1.13.11.52 0.905
-
 lung, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C Meriones unguiculatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 D-tryptophan + O2
-
 Rattus norvegicus N-formyl-D-kynurenine
-
 ?
1.13.11.52 D-tryptophan + O2
-
 Meriones unguiculatus N-formyl-D-kynurenine
-
 ?
1.13.11.52 L-Trp + O2 tryptophan 2,3-dioxygenase activity is appreciable even at 0.03 mM oxygen and rises steeply to a maximum at 0.04 mM Rattus norvegicus L-formylkynurenine
-
 ?
1.13.11.52 L-Trp + O2 indoleamine 2,3-dioxygenase has almost no detectable activity at or below 0.1 mM oxygen and maximum activity at about 1.15 mM Meriones unguiculatus L-formylkynurenine
-
 ?
1.13.11.52 L-Trp + O2 indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid Rattus norvegicus L-formylkynurenine
-
 ?
1.13.11.52 L-Trp + O2 indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid Meriones unguiculatus L-formylkynurenine
-
 ?
1.13.11.52 L-tryptophan + O2 no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen Rattus norvegicus N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2 no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen Meriones unguiculatus N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2-
-
 Rattus norvegicus N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2-
-
 Meriones unguiculatus N-formyl-L-kynurenine
-
 ?