Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Okada, M.; Kawashima, S.; Imahori, K.
    Mode of inactivation of putrescine oxidase by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide or metal ions (1980), J. Biochem., 88, 481-488.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.10 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide the activity of the modified enzyme towards putrescine is 5.6% of that of the native enzyme. The modified enzyme shows activity towards monoamines such as n-butylamine, n-hexylamine and n-octylamine, which are not substrates of the native enzyme Kocuria rosea
1.4.3.10 Cd2+ inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme Kocuria rosea
1.4.3.10 Co2+ inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme Kocuria rosea
1.4.3.10 Cu2+ inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme Kocuria rosea
1.4.3.10 Hg2+ inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme Kocuria rosea
1.4.3.10 Ni2+ inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme Kocuria rosea
1.4.3.10 Zn2+ inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme Kocuria rosea

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.10 Kocuria rosea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.10 additional information the enzyme modified with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide shows activity towards monoamines such as n-butylamine, n-hexylamine and n-octylamine, which are not substrates of the native enzyme Kocuria rosea ?
-
 ?
1.4.3.10 putrescine + O2 + H2O
-
 Kocuria rosea 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.10 spermidine + O2 + H2O
-
 Kocuria rosea 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.10 FAD contains FAD Kocuria rosea