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Literature summary extracted from

  • Yoshimoto, T.; Miyamoto, Y.; Ochi, K.; Yamamoto, S.
    Arachidonate 12-lipoxygenase of porcine leukocyte with activity for 5-hydroxyeicosatetraenoic acid (1982), Biochim. Biophys. Acta, 713, 638-646.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 5,8,11,14-Eicosatetraynoic acid 0.01 mM, 86% inhibition Sus scrofa
1.13.11.31 arachidonic acid inhibits reaction with 5-hydroxy-6,8,11,14-eicodatetraenoic acid Sus scrofa
1.13.11.31 BW755C 0.01 mM, 72% inhibition Sus scrofa
1.13.11.31 nordihydroguaiaretic acid 0.01 mM, 97% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.31 0.0045
-
 5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid
-
 Sus scrofa
1.13.11.31 0.0066
-
 arachidonic acid
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.31 cytosol
-
 Sus scrofa 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 leukocyte polymorphonuclear Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2 40% of the activity with arachidonic acid Sus scrofa 5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
-
 ?
1.13.11.31 5,8,11,14,17-eicosapentaenoic acid + O2 14% of the activity with arachidonic acid Sus scrofa 12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
 ?
1.13.11.31 8,11,14-eicosatrienoic acid + O2 95% of the activity with arachidonic acid Sus scrofa 12-hydroxyeicosatrienoic acid
-
 ?
1.13.11.31 arachidonate + O2 i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid Sus scrofa (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.31 7.5
-
-
 Sus scrofa