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Literature summary extracted from

  • Yokoyama, C.; Mizuno, K.; Mitachi, H.; Yoshimoto, T.; Yamamoto, S.
    Partial purification and characterization of arachidonate 12-lipoxygenase from rat lung (1983), Biochim. Biophys. Acta, 750, 237-243.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 5,8,11,14-Eicosatetraynoic acid 0.001 mM, 77% inhibition Rattus norvegicus
1.13.11.31 8-hydroxyquinoline 1 mM, 35% inhibition Rattus norvegicus
1.13.11.31 BW755C 0.01 mM, 13% inhibition Rattus norvegicus
1.13.11.31 EDTA 1 mM, 40% inhibition Rattus norvegicus
1.13.11.31 nordihydroguaiaretic acid 0.01 mM, 48% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.31 0.005
-
 arachidonic acid
-
 Rattus norvegicus
1.13.11.31 0.03
-
 5,8,11,14,17-eicosapentaenoic acid
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.31 cytosol more than half of the activity Rattus norvegicus 5829
-
1.13.11.31 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 lung
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.31 0.34
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.13.11.31 -70°C, 1 month, no appreciable loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 5,8,11,14,17-eicosapentaenoic acid + O2
-
 Rattus norvegicus 12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
 ?
1.13.11.31 arachidonate + O2 i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid Rattus norvegicus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.31 40
-
 10 min, stable below Rattus norvegicus
1.13.11.31 45
-
 10 min, 60% loss of activity Rattus norvegicus
1.13.11.31 50
-
 10 min, 86% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.31 7.4
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.31 6.1 8.6 pH 6.1: 37% of maximal activity, pH 8.6: 44% of maximal activity Rattus norvegicus