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Literature summary extracted from

  • Rocca, E.; Ghiretti, F.
    Purification and properties of D-glutamic acid oxidase from Octopus vulgaris Lam. (1958), Arch. Biochem. Biophys., 77, 336-349.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.7 ethanol 2fold activation in the presence of ethanol Octopus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.7 iodoacetate 68% inhibition at 0.1 mM Octopus vulgaris
1.4.3.7 o-Iodosobenzoate 32% inhibition at 0.1 mM Octopus vulgaris
1.4.3.7 p-chloromercuribenzoate 74% inhibition at 0.01 mM Octopus vulgaris
1.4.3.7 urethane 31% inhibition at 10 mM Octopus vulgaris
1.4.3.7 Veronal 60% inhibition at 8 mM Octopus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.7 4.5
-
 D-Aspartate
-
 Octopus vulgaris
1.4.3.7 8
-
 D-glutamate
-
 Octopus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.7 D-aspartate + H2O + O2 Octopus vulgaris 70% higher activity with pure oxygen than in air oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.7 D-aspartate + H2O + O2 Octopus vulgaris poorer substrate than D-glutamate oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.7 D-glutamate + H2O + O2 Octopus vulgaris better substrate than D-aspartate 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.7 D-glutamate + H2O + O2 Octopus vulgaris highly specific, 70% higher activity with pure oxygen than in air 2-oxoglutarate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.7 Octopus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.7 to homogeneity, precipitation techniques, calcium phosphate gel treatment Octopus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.7 hepatopancreas
-
 Octopus vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.7 D-aspartate + H2O + O2 70% higher activity with pure oxygen than in air Octopus vulgaris oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.7 D-aspartate + H2O + O2 poorer substrate than D-glutamate Octopus vulgaris oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.7 D-glutamate + H2O + O2 better substrate than D-aspartate Octopus vulgaris 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.7 D-glutamate + H2O + O2 highly specific, 70% higher activity with pure oxygen than in air Octopus vulgaris 2-oxoglutarate + NH3 + H2O2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.7 8.1 8.3
-
 Octopus vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.7 FAD cannot be replaced by FMN Octopus vulgaris