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Literature summary extracted from

  • Tuininga, J.E.; Verhees, C.H.; van der Oost, J.; Kengen, S.W.; Stams, A.J.; de Vos, W.M.
    Molecular and biochemical characterization of the ADP-dependent phosphofructokinase from the hyperthermophilic archaeon Pyrococcus furiosus (1999), J. Biol. Chem., 274, 21023-21028.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.146 expression in Escherichia coli Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.146 AMP competitive inhibition Pyrococcus furiosus
2.7.1.146 ATP competitive inhibition Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.146 Co2+ 81% of activity Pyrococcus furiosus
2.7.1.146 Mg2+ required for activity Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.146 52000
-
 4 * 52000, SDS-PAGE Pyrococcus furiosus
2.7.1.146 180000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.146 D-fructose 6-phosphate + ADP Pyrococcus furiosus ADP can be replaced by GDP, ATP and GTP to a limited extent D-fructose 1,6-bisphosphate + AMP
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.146 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.146 partial Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.146 88
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.146 D-fructose 6-phosphate + ADP ADP can be replaced by GDP, ATP and GTP to a limited extent Pyrococcus furiosus D-fructose 1,6-bisphosphate + AMP
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.1.146 tetramer 4 * 52000, SDS-PAGE Pyrococcus furiosus