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Literature summary extracted from

  • Koga, S.; Yoshioka, I.; Sakuraba, H.; Takahashi, M.; Sakasegawa, S.; Shimizu, S.; Ohshima, T.
    Biochemical characterization, cloning, and sequencing of ADP-dependent (AMP-forming) glucokinase from two hyperthermophilic archaea, Pyrococcus furiosus and Thermococcus litoralis (2000), J. Biochem., 128, 1079-1085.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.147 Co2+ 93% of activity Pyrococcus furiosus
2.7.1.147 Co2+ 22% of activity Thermococcus litoralis
2.7.1.147 Mg2+ required for activation Pyrococcus furiosus
2.7.1.147 Mg2+ required for activation Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.147 47000
-
 2 * 47000 Pyrococcus furiosus
2.7.1.147 52000
-
 1 * 52000 Thermococcus litoralis
2.7.1.147 54000
-
 gel filtration Thermococcus litoralis
2.7.1.147 100000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.147 D-glucose + ADP Pyrococcus furiosus CDP shows comparable activity D-glucose 6-phosphate + AMP
-
 ir
2.7.1.147 D-glucose + ADP Thermococcus litoralis CDP shows comparable activity D-glucose 6-phosphate + AMP
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.147 Pyrococcus furiosus
-
-
-
2.7.1.147 Thermococcus litoralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.147
-
 Pyrococcus furiosus
2.7.1.147
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.147 166
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.147 D-1,5-anhydroglucitol + ADP
-
 Pyrococcus furiosus ?
-
 ?
2.7.1.147 D-1,5-anhydroglucitol + ADP
-
 Thermococcus litoralis ?
-
 ?
2.7.1.147 D-glucose + ADP CDP shows comparable activity Pyrococcus furiosus D-glucose 6-phosphate + AMP
-
 ir
2.7.1.147 D-glucose + ADP CDP shows comparable activity Thermococcus litoralis D-glucose 6-phosphate + AMP
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.1.147 dimer 2 * 47000 Pyrococcus furiosus
2.7.1.147 monomer 1 * 52000 Thermococcus litoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.147 90
-
-
 Thermococcus litoralis
2.7.1.147 90
-
 at least stable for 10 min Pyrococcus furiosus