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Literature summary extracted from

  • Kengen, S.W.M.; Tuininga, J.E.; Verhees, C.H.; Van der Oost, J.; Stams, A.J.M.; De Vos, W.M.
    ADP-dependent glucokinase and phosphofructokinase from Pyrococcus furiosus (2001), Methods Enzymol., 331, 41-53.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.146 expression in Escherichia coli Pyrococcus furiosus
2.7.1.147 expression in Escherichia coli Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.146 AMP competitive inhibition Pyrococcus furiosus
2.7.1.146 ATP competitive inhibition Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.146 Co2+ 81% of activity Pyrococcus furiosus
2.7.1.146 Mg2+ required for activity Pyrococcus furiosus
2.7.1.147 Ca2+ 17% of activity Pyrococcus furiosus
2.7.1.147 Mg2+ required for activation Pyrococcus furiosus
2.7.1.147 Mn2+ 77% of activity Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.146 52000
-
 4 * 52000, SDS-PAGE Pyrococcus furiosus
2.7.1.146 180000
-
 gel filtration Pyrococcus furiosus
2.7.1.147 47000
-
 2 * 47000 Pyrococcus furiosus
2.7.1.147 93000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.146 D-fructose 6-phosphate + ADP Pyrococcus furiosus ADP can be replaced by GDP, ATP and GTP to a limited extent D-fructose 1,6-bisphosphate + AMP
-
 ?
2.7.1.147 D-glucose + ADP Pyrococcus furiosus
-
 D-glucose 6-phosphate + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.146 Pyrococcus furiosus
-
-
-
2.7.1.147 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.146 partial Pyrococcus furiosus
2.7.1.147
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.147 307
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.146 D-fructose 6-phosphate + ADP ADP can be replaced by GDP, ATP and GTP to a limited extent Pyrococcus furiosus D-fructose 1,6-bisphosphate + AMP
-
 ?
2.7.1.147 D-glucose + ADP
-
 Pyrococcus furiosus D-glucose 6-phosphate + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.146 tetramer 4 * 52000, SDS-PAGE Pyrococcus furiosus
2.7.1.147 dimer 2 * 47000 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.147 100
-
 stable for 220 min Pyrococcus furiosus