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Literature summary extracted from
- Cytochrome cb-type nitric oxide reductase with cytochrome c oxidase activity from Paracoccus denitrificans ATCC 35512 (1996), J. Bacteriol., 178, 1866-1871.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.2.5 | KCN | 50% inhibition at 0.3 mM | Paracoccus denitrificans |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.7.2.5 | 0.00025 | - |
NO | - |
Paracoccus denitrificans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.2.5 | Fe | contains 24.9 nmol heme b and 14.2 nmol heme c per mg protein | Paracoccus denitrificans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.7.2.5 | 15000 | - |
1 * 34000 + 1 * 15000, SDS-PAGE | Paracoccus denitrificans |
| 1.7.2.5 | 34000 | - |
1 * 34000 + 1 * 15000, SDS-PAGE | Paracoccus denitrificans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.2.5 | 2 nitric oxide + reduced acceptor | Paracoccus denitrificans | - |
nitrous oxide + acceptor + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.7.2.5 | Paracoccus denitrificans | - |
strain ATCC 35512 formerly Thiosphaera pantotropha | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.7.2.5 | - |
Paracoccus denitrificans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.2.5 | 2 nitric oxide + reduced acceptor | - |
Paracoccus denitrificans | nitrous oxide + acceptor + H2O | - |
? | |
| 1.7.2.5 | 2 nitric oxide + reduced acceptor | ferrocytochrome c-550 as electron donor | Paracoccus denitrificans | nitrous oxide + acceptor + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.7.2.5 | dimer | 1 * 34000 + 1 * 15000, SDS-PAGE | Paracoccus denitrificans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.2.5 | heme | - |
Paracoccus denitrificans | |
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