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Literature summary extracted from
- Characterization of a NADPH-dependent coenzyme A-SS-glutathione reductase from yeast (1969), Biochim. Biophys. Acta, 191, 239-248.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.10 | phosphate | 25 mM, partially inhibits | Saccharomyces cerevisiae |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.10 | 0.2 | - |
CoA-glutathione | at pH 5.5 and a fixed value of 110 nmol NADPH | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.10 | 108000 | - |
sucrose density gradient ultracentrifugation | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.10 | Saccharomyces cerevisiae | - |
strain ATCC 1946 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.10 | 143fold partial purification, separated from GSSG reductase activity, EC 1.6.4.2 | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.10 | additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.10 | CoA-glutathione + NADPH | - |
Saccharomyces cerevisiae | glutathione + CoA + NADP+ | - |
ir | |
| 1.8.1.10 | additional information | no reduction of CoASSCys, GSSCys or CysSSCys | Saccharomyces cerevisiae | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.10 | 24 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.10 | 5.5 | - |
- |
Saccharomyces cerevisiae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.10 | NADPH | NADPH-dependent | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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