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Literature summary extracted from

  • Braks, I.J.; Hoppert, M.; Roge, S.; Mayer, F.
    Structural aspects and immunolocalization of the F420-reducing and non-F420-reducing hydrogenases from Methanobacterium thermoautotrophicum Marburg (1994), J. Bacteriol., 176, 7677-7687.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.98.1 membrane associated to cytoplasmatic membrane and to inside-out membrane vesicles, attached to membrane by linker peptide of 10000-20000 Da Methanothermobacter thermautotrophicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.1 790000
-
native SDS-PAGE, sucrose density centrifugation Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanothermobacter thermautotrophicus enzyme of methanogenesis pathway reduced coenzyme F420
-
?
1.12.98.1 H2 + coenzyme F420 Methanothermobacter thermautotrophicus Marburg / DSM 2133 enzyme of methanogenesis pathway reduced coenzyme F420
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanothermobacter thermautotrophicus
-
-
-
1.12.98.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.1
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + coenzyme F420
-
Methanothermobacter thermautotrophicus reduced coenzyme F420
-
?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanothermobacter thermautotrophicus reduced coenzyme F420
-
?
1.12.98.1 H2 + coenzyme F420
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133 reduced coenzyme F420
-
?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanothermobacter thermautotrophicus Marburg / DSM 2133 reduced coenzyme F420
-
?
1.12.98.1 H2 + methyl viologen
-
Methanothermobacter thermautotrophicus ?
-
?
1.12.98.1 H2 + methyl viologen
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 multimer alpha,beta,gamma 48000 + 33000 + 27000, SDS-PAGE, exact subunit composition not known Methanothermobacter thermautotrophicus