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Literature summary extracted from

  • Shah, N.N.; Clark, D.S.
    Partial purification and characterization of two hydrogenases from the extreme thermophile Methanococcus jannaschii (1990), Appl. Environ. Microbiol., 56, 858-863.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.98.1 additional information 2 times higher activity in biological buffers like Tris or EPPS compared with phosphate buffer Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.1 115000
-
 smallest species with enzymatic activity, native SDS-PAGE Methanocaldococcus jannaschii
1.12.98.1 990000
-
 native SDS-PAGE Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanocaldococcus jannaschii enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.1 partial Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.1 5.2
-
 purified enzyme with F420 Methanocaldococcus jannaschii
1.12.98.1 16
-
 purified enzyme with methyl viologen Methanocaldococcus jannaschii

Storage Stability

EC Number Storage Stability Organism
1.12.98.1 -70°C, loss of high temperature activity Methanocaldococcus jannaschii
1.12.98.1 4°C, 5% loss of activity within 24 h Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + coenzyme F420
-
 Methanocaldococcus jannaschii reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanocaldococcus jannaschii reduced coenzyme F420
-
 ?
1.12.98.1 H2 + methyl viologen
-
 Methanocaldococcus jannaschii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 multimer alpha,beta,gamma 48000 + 32000 + 25000, SDS-PAGE, exact subunit composition not known Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.98.1 80 90
-
 Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.12.98.1 25 90 F420 reduction Methanocaldococcus jannaschii
1.12.98.1 40 103 at 103°C low activity with methyl viologen Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.98.1 70
-
 stable for more than 3 h, methyl viologen-linked activity more stable against heating than F420-linked activity Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.1 7
-
 F420 reduction Methanocaldococcus jannaschii
1.12.98.1 10
-
 optimum pH for methyl viologen reduction above pH 10 Methanocaldococcus jannaschii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.12.98.1 10
-
 unstable above pH 10 Methanocaldococcus jannaschii