Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Muth, E.; Mörschel, E.; Klein, A.
    Purification and characterization of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from the archaebacterium Methanococcus voltae (1987), Eur. J. Biochem., 169, 571-577.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.12.98.1 FAD stabilizes F420-linked activity against heat inactivation, generally stabilizing Methanococcus voltae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.98.1 0.016
-
 coenzyme F420
-
 Methanococcus voltae
1.12.98.1 1.2
-
 methyl viologen
-
 Methanococcus voltae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.98.1 soluble
-
 Methanococcus voltae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.1 Iron
-
 Methanococcus voltae
1.12.98.1 Nickel
-
 Methanococcus voltae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.1 105000
-
 smallest active species, gel filtration, sucrose density centrifugation Methanococcus voltae
1.12.98.1 745000
-
 gel filtration, sucrose density centrifugation Methanococcus voltae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanococcus voltae enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanococcus voltae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.1
-
 Methanococcus voltae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.1 10
-
 purified enzyme, F420-linked activity Methanococcus voltae
1.12.98.1 50
-
 purified enzyme, methyl viologen-linked activity Methanococcus voltae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + coenzyme F420
-
 Methanococcus voltae reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanococcus voltae reduced coenzyme F420
-
 ?
1.12.98.1 H2 + methyl viologen binding site for methyl viologen different from F420 binding site as determined by blocking of binding site with antibodies against native enzyme Methanococcus voltae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 multimer alpha,beta,gamma,delta 55000 + 45000 + 37000 + 27000, SDS-PAGE, exact subunit composition not known, function of delta subunit not known Methanococcus voltae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.98.1 37
-
-
 Methanococcus voltae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.98.1 65
-
 methyl viologen-reducing activity heat resistent up to 65°C, F420-reducing activity 75% inactivated at 65°C Methanococcus voltae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.98.1 17.5
-
 coenzyme F420
-
 Methanococcus voltae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.1 7
-
 F420 reduction Methanococcus voltae
1.12.98.1 7.5
-
 methyl viologen reduction Methanococcus voltae

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.98.1 FAD 1 mol FAD per mol 105000 Da species Methanococcus voltae