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Literature summary extracted from

  • Nakai, C.; Nakazawa, T.; Nozaki, M.
    Purification and properties of catechol 1,2-dioxygenase (pyrocatechase) from Pseudomonas putida mt-2 in comparison with that from Pseudomonas arvilla C-1 (1988), Arch. Biochem. Biophys., 267, 701-713.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 p-chloromercuribenzoate 64% inactivation, 5 mM, 30 min, 24°C Pseudomonas putida
1.13.11.1 Tiron 48% inactivation, 5 mM, 30 min, 24°C Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.002
-
 catechol
-
 Pseudomonas putida
1.13.11.1 0.06
-
 oxygen
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron 1.1 gatom per mol enzyme protein based on a molecular weight of 65000 Da Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 32000
-
 2 * 32000, SDS-PAGE Pseudomonas putida
1.13.11.1 64000 66000 gel filtration, sedimentation equilibrium analysis Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Pseudomonas putida
-
 mt-2, ATCC 23973, TN2100
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 additional information
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.13.11.1 4°C, pH 8.5, stable over a period of several months Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2 poor substrate Pseudomonas putida (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-chlorocatechol + O2 poor substrate Pseudomonas putida (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Pseudomonas putida (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 catechol + O2
-
 Pseudomonas putida cis,cis-muconate
-
 ?
1.13.11.1 additional information no substrates: 4-nitrocatechol, protocatechuic acid, ratios of intradiol and extradiol cleavage activities for 3-methylcatechol and 3-methoxycatechol 22 : 4.3 Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 dimer 2 * 32000, SDS-PAGE Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7.5
-
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.1 8 9
-
 Pseudomonas putida