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Literature summary extracted from

  • Chen, Y.P.; Glenn, A.R.; Dilworth, M.J.
    Aromatic metabolism in Rhizobium trifolii: catechol 1,2-dioxygenase (1985), Arch. Microbiol., 141, 225-228.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 1,10-phenanthroline progressive inactivation, loss of all activation after 90 min, reactivation with Fe3+ Rhizobium leguminosarum
1.13.11.1 Al3+ 0.01 M, 100% Rhizobium leguminosarum
1.13.11.1 KCN
-
 Rhizobium leguminosarum
1.13.11.1 Ni2+ 0.01 M, 85% Rhizobium leguminosarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.0022
-
 catechol
-
 Rhizobium leguminosarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron 2 gatom per mol enzyme protein Rhizobium leguminosarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 59000
-
 2 * 59000, SDS-PAGE Rhizobium leguminosarum
1.13.11.1 107000
-
 gel filtration Rhizobium leguminosarum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Rhizobium leguminosarum
-
 TA1
-
1.13.11.1 Rhizobium leguminosarum TA1
-
 TA1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Rhizobium leguminosarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 6.25
-
-
 Rhizobium leguminosarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 4-methylcatechol + O2
-
 Rhizobium leguminosarum (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Rhizobium leguminosarum TA1 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 catechol + O2
-
 Rhizobium leguminosarum cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Rhizobium leguminosarum TA1 cis,cis-muconate
-
 ?
1.13.11.1 additional information no substrates: 3,4-dihydroxybenzoate, 3-methoxy-4-hydroxybenzoate, 2,4-dihydroxybenzoate, 3,5-dihydroxybenzoate, 3,4,5-trihydroxybenzoate Rhizobium leguminosarum ?
-
 ?
1.13.11.1 additional information no substrates: 3,4-dihydroxybenzoate, 3-methoxy-4-hydroxybenzoate, 2,4-dihydroxybenzoate, 3,5-dihydroxybenzoate, 3,4,5-trihydroxybenzoate Rhizobium leguminosarum TA1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 dimer 2 * 59000, SDS-PAGE Rhizobium leguminosarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7 9
-
 Rhizobium leguminosarum