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Literature summary extracted from

  • Aoki, K.; Konohana, T.; Shinke, R.; Nishira, H.
    Two catechol 1,2-dioxygenases from aniline-assimilating bacterium, Frateuria species ANA-18 (1984), Agric. Biol. Chem., 48, 2097-2104.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.1 Fe2+ CD I and CD II respectively Frateuria sp.
1.13.11.1 Fe3+ CD I and CD II respectively Frateuria sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 Ag+ CD I only Frateuria sp.
1.13.11.1 Hg2+ CD I only Frateuria sp.
1.13.11.1 iodoacetic acid CD I only Frateuria sp.
1.13.11.1 o-phenanthroline CD I and CD II respectively Frateuria sp.
1.13.11.1 p-chloromercuribenzoate CD I only Frateuria sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron 1.2 gatom per mol enzyme protein based on a molecular weight of 38000 and 36000 for CD I and CD II respecetively Frateuria sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 36000
-
 CD II: 4 * 36000, SDS-PAGE Frateuria sp.
1.13.11.1 37000
-
 both CD I and CD II, buffer containing NaCl, gel filtration Frateuria sp.
1.13.11.1 38000
-
 CD I: 4 * 38000, SDS-PAGE Frateuria sp.
1.13.11.1 140000
-
 buffer omitting NaCl, gel filtration Frateuria sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Frateuria sp.
-
 ANA-18
-
1.13.11.1 Frateuria sp. ANA-18
-
 ANA-18
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 CD I and CD II respectively Frateuria sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 additional information
-
-
 Frateuria sp.

Storage Stability

EC Number Storage Stability Organism
1.13.11.1 6°C, buffer containing 0.1 M NaCl, 50 days without decrease of activity Frateuria sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2 poor substrate Frateuria sp. (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 3-methylcatechol + O2 poor substrate Frateuria sp. ANA-18 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2 poor substrate Frateuria sp. (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2 poor substrate Frateuria sp. ANA-18 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 catechol + O2
-
 Frateuria sp. cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Frateuria sp. ANA-18 cis,cis-muconate
-
 ?
1.13.11.1 additional information ratios of intradiol to extradiol activity of CD I and CD II 1000:120 and 100:3 respectively Frateuria sp. ?
-
 ?
1.13.11.1 additional information ratios of intradiol to extradiol activity of CD I and CD II 1000:120 and 100:3 respectively Frateuria sp. ANA-18 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 tetramer CD I: 4 * 38000, SDS-PAGE Frateuria sp.
1.13.11.1 tetramer CD II: 4 * 36000, SDS-PAGE Frateuria sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.1 35
-
 for 10 min, stable activity of CD I, activity of CD II decrease to 89% Frateuria sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7.5
-
 for CD I and CD II respectively Frateuria sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 6.5 10 for CD I and CD II respectively Frateuria sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.1 5 10.5 5°C, 24 hours Frateuria sp.