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Literature summary extracted from

  • Patel, R.N.; Hou, C.T.; Felix, A.; Lillard, M.O.
    Catechol 1,2-dioxygenase from Acinetobacter calcoaceticus: purification and properties (1976), J. Bacteriol., 127, 536-544.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 2-mercaptoethanol
-
 Acinetobacter calcoaceticus
1.13.11.1 3-Isopropylcatechol
-
 Acinetobacter calcoaceticus
1.13.11.1 3-methylcatechol
-
 Acinetobacter calcoaceticus
1.13.11.1 4-methylcatechol
-
 Acinetobacter calcoaceticus
1.13.11.1 4-nitrocatechol
-
 Acinetobacter calcoaceticus
1.13.11.1 Ag+
-
 Acinetobacter calcoaceticus
1.13.11.1 alpha-chloro-3,4-dihydroxyacetophenone
-
 Acinetobacter calcoaceticus
1.13.11.1 Cu2+
-
 Acinetobacter calcoaceticus
1.13.11.1 p-hydroxymercuribenzoate
-
 Acinetobacter calcoaceticus
1.13.11.1 phenol
-
 Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron 2 gatom per mol enzyme protein Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 40000
-
 1 * 40000, SDS-PAGE Acinetobacter calcoaceticus
1.13.11.1 81000 85000 sedimentation equilibrium analysis, gel filtration Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Acinetobacter calcoaceticus
-
-
-
1.13.11.1 Acinetobacter calcoaceticus ADP-96
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 20
-
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-isopropylcatechol + O2
-
 Acinetobacter calcoaceticus 2-isopropyl-cis,cis-muconate
-
 ?
1.13.11.1 3-isopropylcatechol + O2
-
 Acinetobacter calcoaceticus ADP-96 2-isopropyl-cis,cis-muconate
-
 ?
1.13.11.1 3-methylcatechol + O2
-
 Acinetobacter calcoaceticus (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 3-methylcatechol + O2
-
 Acinetobacter calcoaceticus ADP-96 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Acinetobacter calcoaceticus (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Acinetobacter calcoaceticus ADP-96 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 catechol + O2
-
 Acinetobacter calcoaceticus cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Acinetobacter calcoaceticus ADP-96 cis,cis-muconate
-
 ?
1.13.11.1 additional information no substrates: 4-nitrocatechol, alpha-chloro-3,4-dihydroxyacetophenone, 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxycinnamic acid, protocatechuic acid, protoacatechualdehyde, pyrogallol Acinetobacter calcoaceticus ?
-
 ?
1.13.11.1 additional information no substrates: 4-nitrocatechol, alpha-chloro-3,4-dihydroxyacetophenone, 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxycinnamic acid, protocatechuic acid, protoacatechualdehyde, pyrogallol Acinetobacter calcoaceticus ADP-96 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 dimer 1 * 40000, SDS-PAGE Acinetobacter calcoaceticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 35 37
-
 Acinetobacter calcoaceticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.1 43
-
 30 min, 18% loss of activity Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7 9
-
 Acinetobacter calcoaceticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.1 6
-
 complete loss of activity after 6 days Acinetobacter calcoaceticus