Literature summary extracted from
Gorlatov, S.N.; Stadtman, T.C.
Human selenium-dependent thioredoxin reductase from HeLa cells: properties of forms with differing heparin affinities (1999), Arch. Biochem. Biophys., 369, 133-142.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.8.1.9 |
phosphate |
under growth conditions |
Homo sapiens |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.8.1.9 |
cytosol |
isoform TrxR1 |
Homo sapiens |
5829 |
- |
1.8.1.9 |
mitochondrion |
isoform TrxR2 |
Homo sapiens |
5739 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.8.1.9 |
selenocysteine |
heparin affinity depends on the selenium content, binding can be induced by reduction of the enzyme with NADPH or tris-(2-carboxyethyl)phosphine |
Homo sapiens |
|
1.8.1.9 |
selenocysteine |
contains selenocysteine as part of the redox active selenosulfide in the active center |
Homo sapiens |
|
1.8.1.9 |
selenocysteine |
isoform 1 contains 1 selenium per subunit, the mitochondrial isoform 2 contains 0.5 selenium per subunit |
Homo sapiens |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.8.1.9 |
54800 |
- |
2 * 54800, HeLa cell enzyme, MALDI |
Homo sapiens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.8.1.9 |
Homo sapiens |
- |
HeLa cells |
- |
Oxidation Stability
EC Number |
Oxidation Stability |
Organism |
---|
1.8.1.9 |
the reduced enzyme is very labile to O2, decrease of selenium content and activity after exposure to air, protection by NADPH, NADP+ and tris-(2-carboxyethyl)phosphine |
Homo sapiens |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.8.1.9 |
heparin affinity depends on the selenium content, binding can be induced by reduction of the enzyme with NADPH or tris-(2-carboxyethyl)phosphine |
Homo sapiens |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.8.1.9 |
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ |
conformational change upon reduction wth NADPH or tris-(2-carboxyethyl)phosphine |
Homo sapiens |
|
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.8.1.9 |
HeLa cell |
high and low affinity form with respect to heparin |
Homo sapiens |
- |
1.8.1.9 |
HeLa cell |
heparin affinity depends on the selenium content |
Homo sapiens |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.8.1.9 |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH |
i.e. DTNB |
Homo sapiens |
2-nitro-5-thiobenzoate + NADP+ |
- |
? |
|
1.8.1.9 |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH |
coupled assay |
Homo sapiens |
2-nitro-5-thiobenzoate + NADP+ |
- |
? |
|
1.8.1.9 |
thioredoxin + NADP+ |
coupled assay with DTNB |
Homo sapiens |
thioredoxin disulfide + NADPH |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.8.1.9 |
dimer |
2 * 54800, HeLa cell enzyme, MALDI |
Homo sapiens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.8.1.9 |
TrxR |
- |
Homo sapiens |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.8.1.9 |
FAD |
- |
Homo sapiens |
|